A Functional Assay to Determine the Capacity of Oncolytic Viruses to Induce Immunogenic Tumor Cell Death

Oncolytic immunotherapy efficacy relies partially on the induction of immunogenic tumor cell death following infection with oncolytic viruses (OV) to induce an antitumor immune response. Here, we describe a method to determine if an OV is able to induce such an immunogenic tumor cell death. This method consists in testing whether tumor cells lysed by an OV are able to induce the maturation of human monocyte-derived immature dendritic cells (Mo-iDC).

Mots clés

Antigen-presenting cells Dendritic cells Flow cytometry Immunogenic cell death Oncolytic viruses

Domaines

Cancer

Elizabeth Bernardo : Connectez-vous pour contacter le contributeur

https://inserm.hal.science/inserm-02285959

Soumis le : vendredi 13 septembre 2019-11:45:45

Dernière modification le : lundi 4 mars 2024-13:36:06

Dates et versions

inserm-02285959 , version 1 (13-09-2019)

Identifiants

HAL Id : inserm-02285959 , version 1
DOI : 10.1007/978-1-4939-9794-7_8
PUBMED : 31486035

Citer

Tiphaine Delaunay, Carole Achard, Marc Gregoire, Frédéric Tangy, Nicolas Boisgerault, et al.. A Functional Assay to Determine the Capacity of Oncolytic Viruses to Induce Immunogenic Tumor Cell Death. Methods in Molecular Biology, 2020, 2058, pp.127-132. ⟨10.1007/978-1-4939-9794-7_8⟩. ⟨inserm-02285959⟩

Exporter

BibTeX XML-TEI Dublin Core DC Terms EndNote DataCite

Collections

INSERM PASTEUR UNIV-NANTES CNRS UNIV-ANGERS CRCINA NANTES-UNIVERSITE PASTEUR_UMR3569 UFR-MEDECINE-NANTES

275 Consultations

0 Téléchargements