A Functional Assay to Determine the Capacity of Oncolytic Viruses to Induce Immunogenic Tumor Cell Death

Oncolytic immunotherapy efficacy relies partially on the induction of immunogenic tumor cell death following infection with oncolytic viruses (OV) to induce an antitumor immune response. Here, we describe a method to determine if an OV is able to induce such an immunogenic tumor cell death. This method consists in testing whether tumor cells lysed by an OV are able to induce the maturation of human monocyte-derived immature dendritic cells (Mo-iDC).

Keywords

Antigen-presenting cells Dendritic cells Flow cytometry Immunogenic cell death Oncolytic viruses

Domains

Cancer

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https://www.hal.inserm.fr/inserm-02285959

Submitted on : Friday, September 13, 2019-11:45:45 AM

Last modification on : Saturday, April 22, 2023-8:16:03 AM

Dates and versions

inserm-02285959 , version 1 (13-09-2019)

Identifiers

HAL Id : inserm-02285959 , version 1
DOI : 10.1007/978-1-4939-9794-7_8
PUBMED : 31486035

Cite

Tiphaine Delaunay, Carole Achard, Marc Gregoire, Frédéric Tangy, Nicolas Boisgerault, et al.. A Functional Assay to Determine the Capacity of Oncolytic Viruses to Induce Immunogenic Tumor Cell Death. Methods in Molecular Biology, 2020, 2058, pp.127-132. ⟨10.1007/978-1-4939-9794-7_8⟩. ⟨inserm-02285959⟩

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