A Functional Assay to Determine the Capacity of Oncolytic Viruses to Induce Immunogenic Tumor Cell Death

Tiphaine Delaunay 1, 2 Carole Achard 1, 2 Marc Gregoire 1, 2 Frédéric Tangy 3 Nicolas Boisgerault 1, 2 Jean-François Fonteneau 1, 2, *
* Corresponding author
1 CRCINA - Département INCIT - Equipe 4 - Mort cellulaire immunogénique appliquée aux traitements du mésothéliome
CRCINA - Centre de recherche de Cancérologie et d'Immunologie / Nantes - Angers
2 LabEX IGO Immunothérapie Grand Ouest
3 Génomique virale et vaccination
Abstract : Oncolytic immunotherapy efficacy relies partially on the induction of immunogenic tumor cell death following infection with oncolytic viruses (OV) to induce an antitumor immune response. Here, we describe a method to determine if an OV is able to induce such an immunogenic tumor cell death. This method consists in testing whether tumor cells lysed by an OV are able to induce the maturation of human monocyte-derived immature dendritic cells (Mo-iDC).
Keywords : Antigen-presenting cells Dendritic cells Flow cytometry Immunogenic cell death Oncolytic viruses
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Journal articles
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https://www.hal.inserm.fr/inserm-02285959
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Submitted on : Friday, September 13, 2019 - 11:45:45 AM
Last modification on : Saturday, September 14, 2019 - 1:40:51 AM

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Tiphaine Delaunay, Carole Achard, Marc Gregoire, Frédéric Tangy, Nicolas Boisgerault, et al.. A Functional Assay to Determine the Capacity of Oncolytic Viruses to Induce Immunogenic Tumor Cell Death. Methods in Molecular Biology, Humana Press/Springer Imprint, 2020, 2058, pp.127-132. ⟨10.1007/978-1-4939-9794-7_8⟩. ⟨inserm-02285959⟩

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