A Functional Assay to Determine the Capacity of Oncolytic Viruses to Induce Immunogenic Tumor Cell Death

Tiphaine Delaunay; Carole Achard; Marc Gregoire; Frédéric Tangy; Nicolas Boisgerault; Jean-François Fonteneau

doi:10.1007/978-1-4939-9794-7_8

Journal articles

A Functional Assay to Determine the Capacity of Oncolytic Viruses to Induce Immunogenic Tumor Cell Death

Tiphaine Delaunay ^{1, 2} Carole Achard ^{1, 2} Marc Gregoire ^{1, 2} Frédéric Tangy ³ Nicolas Boisgerault ^{1, 2} Jean-François Fonteneau ^{1, 2, *}

* Corresponding author

1 CRCINA - Département INCIT - Equipe 4 - Mort cellulaire immunogénique appliquée aux traitements du mésothéliome

CRCINA - Centre de recherche de Cancérologie et d'Immunologie / Nantes - Angers

2 LabEX IGO Immunothérapie Grand Ouest

3 Génomique virale et vaccination

Abstract : Oncolytic immunotherapy efficacy relies partially on the induction of immunogenic tumor cell death following infection with oncolytic viruses (OV) to induce an antitumor immune response. Here, we describe a method to determine if an OV is able to induce such an immunogenic tumor cell death. This method consists in testing whether tumor cells lysed by an OV are able to induce the maturation of human monocyte-derived immature dendritic cells (Mo-iDC).

Keywords : Antigen-presenting cells Dendritic cells Flow cytometry Immunogenic cell death Oncolytic viruses

Domain :

Life Sciences [q-bio] / Cancer

Complete list of metadatas

https://www.hal.inserm.fr/inserm-02285959
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Submitted on : Friday, September 13, 2019 - 11:45:45 AM
Last modification on : Thursday, February 20, 2020 - 2:28:06 PM

A Functional Assay to Determine the Capacity of Oncolytic Viruses to Induce Immunogenic Tumor Cell Death

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A Functional Assay to Determine the Capacity of Oncolytic Viruses to Induce Immunogenic Tumor Cell Death

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