Viral and tumor antigen-specific CD8 T-cell responses in Merkel cell carcinoma

Abstract : Merkel cell carcinoma (MCC) is a rare and aggressive cutaneous cancer, which is immunogenic, regardless of the presence of MCPyV (80% of cases). The identification of MCC-specific epitopes recognized by CD8 T cells is crucial to expand the arsenal of immunotherapeutic treatments. Until now, most efforts focused on the identification of virus-specific epitopes, whereas immune responses directed against shared cellular tumor-specific antigens have not been evidenced. In this study, we measured T-cell responses against viral (n = 3) and tumor antigens (n = 47) from TILs derived from 21 MCC tumors. Virus-specific CD8 T-cell responses dominated MCC-specific immune responses, and we identified two new HLA-peptide complexes derived from the LT antigen, located in a region encompassing 3 previously identified epitopes. Finally, we show that MAGE-A3 antigen, frequently expressed by MCC tumors, was recognized by CD8 TILs from a virus-negative MCC tumor and thus could be a target for immunotherapy in this setting.
Complete list of metadatas

Cited literature [51 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-02284748
Contributor : Nathalie Labarriere <>
Submitted on : Thursday, September 12, 2019 - 10:17:07 AM
Last modification on : Friday, September 13, 2019 - 1:25:13 AM

File

 Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed until : jamais

Please log in to resquest access to the document

Identifiers

Citation

Mahtab Samimi, Houssem Benlalam, Pascal Aumond, Pauline Gaboriaud, Delphine Fradin, et al.. Viral and tumor antigen-specific CD8 T-cell responses in Merkel cell carcinoma. Cellular Immunology, Elsevier, 2019, 344, pp.103961. ⟨10.1016/j.cellimm.2019.103961⟩. ⟨inserm-02284748⟩

Share

Metrics

Record views

48