Why Are Botulinum Neurotoxin-Producing Bacteria So Diverse and Botulinum Neurotoxins So Toxic?, Toxins (Basel), issue.1, p.11, 2019. ,
URL : https://hal.archives-ouvertes.fr/hal-02328868
The life history of a botulinum toxin molecule, Toxicon, vol.68, pp.40-59, 2013. ,
Botulinum neurotoxins: mechanism of action, Toxicon, vol.67, pp.87-93, 2013. ,
, , 2001.
Botulinum neurotoxins: new questions arising from structural biology, Trends Biochem Sci, vol.39, issue.11, pp.517-526, 2014. ,
The pre-synaptic motor nerve terminal as a site for antibody-mediated neurotoxicity in autoimmune neuropathies and synaptopathies, J Anat, vol.224, issue.1, pp.36-44, 2014. ,
A hitchhiker's guide to the nervous system: the complex journey of viruses and toxins, Nat Rev Microbiol, vol.8, issue.9, pp.645-655, 2010. ,
Brain Lipids in Synaptic Function and Neurological Disease : Clues to Innovative Therapeutic Strategies for Brain Disorders, 2015. ,
Gangliosides of the Vertebrate Nervous System, Journal of Molecular Biology, vol.428, issue.16, pp.3325-3336, 2016. ,
Structural basis of cell surface receptor recognition by botulinum neurotoxin B, Nature, vol.444, issue.7122, pp.1096-1100, 2006. ,
Presynaptic receptor arrays for clostridial neurotoxins, Trends Microbiol, vol.12, issue.10, pp.442-446, 2004. ,
Membrane lipid domains in the nervous system, Front Biosci, vol.20, pp.280-302, 2015. ,
SNARE proteins are highly enriched in lipid rafts in PC12 cells: implications for the spatial control of exocytosis, Proc Natl Acad Sci U S A, vol.98, issue.10, pp.5619-5624, 2001. ,
Uncoupling the roles of synaptotagmin I during endo-and exocytosis of synaptic vesicles, Nat Neurosci, vol.15, issue.2, pp.243-249, 2011. ,
Glycosylation is dispensable for sorting of synaptotagmin 1 but is critical for targeting of SV2 and synaptophysin to recycling synaptic vesicles, J Biol Chem, vol.287, issue.42, pp.35658-35668, 2012. ,
A Ca(2+) Sensor for Exocytosis, Trends Neurosci, vol.41, issue.6, pp.327-330, 2018. ,
Botulinum neurotoxin B recognizes its protein receptor with high affinity and specificity, Nature, vol.444, issue.7122, pp.1092-1095, 2006. ,
Botulinum neurotoxin D-C uses synaptotagmin I and II as receptors, and human synaptotagmin II is not an effective receptor for type B, D-C and G toxins, J Cell Sci, vol.125, pp.3233-3242, 2012. ,
Engineered botulinum neurotoxin B with improved binding to human receptors has enhanced efficacy in preclinical models, Sci Adv, vol.5, issue.1, p.7196, 2019. ,
Structure of dual receptor binding to botulinum neurotoxin B, Nat Commun, vol.4, 2013. ,
How do tetanus and botulinum toxins bind to neuronal membranes?, Trends Biochem Sci, vol.11, issue.8, pp.314-317, 1986. ,
Structural biology: dangerous liaisons on neurons, Nature, vol.444, issue.7122, pp.1019-1020, 2006. ,
A lipid-binding loop of botulinum neurotoxin serotypes B, DC and G is an essential feature to confer their exquisite potency, PLoS Pathog, vol.14, issue.5, p.1007048, 2018. ,
Affinity biosensors using recombinant native membrane proteins displayed on exosomes: application to botulinum neurotoxin B receptor, Sci Rep, vol.7, issue.1, p.1032, 2017. ,
URL : https://hal.archives-ouvertes.fr/inserm-01513204
Prediction of glycolipid-binding domains from the amino acid sequence of lipid raft-associated proteins: application to HpaA, a protein involved in the adhesion of Helicobacter pylori to gastrointestinal cells, Biochemistry, vol.45, issue.36, pp.10957-10962, 2006. ,
Molecular basis for the glycosphingolipid-binding specificity of alpha-synuclein: key role of tyrosine 39 in membrane insertion, Journal of Molecular Biology, vol.408, issue.4, pp.654-669, 2011. ,
Greasing their way: lipid modifications determine protein association with membrane rafts, Biochemistry, vol.49, issue.30, pp.6305-6316, 2010. ,
Interaction of peptides with binary phospholipid membranes: application of fluorescence methodologies, Chem Phys Lipids, vol.122, issue.1-2, pp.77-96, 2003. ,
Ionization potentials of fluoroindoles and the origin of nonexponential tryptophan fluorescence decay in proteins, J Am Chem Soc, vol.127, issue.11, pp.4104-4113, 2005. ,
Engineered botulinum neurotoxin B with improved efficacy for targeting human receptors, Nat Commun, vol.8, issue.1, p.53, 2017. ,
Electric dipole reorientation in the interaction of botulinum neurotoxins with neuronal membranes, FEBS Lett, vol.583, issue.14, pp.2321-2325, 2009. ,
Synaptotagmins I and II mediate entry of botulinum neurotoxin B into cells, J Cell Biol, vol.162, issue.7, pp.1293-1303, 2003. ,
Botulinum Neurotoxins Can Enter Cultured Neurons Independent of Synaptic Vesicle Recycling, PLoS One, vol.10, issue.7, p.133737, 2015. ,
Mechanism of botulinum neurotoxin B and G entry into hippocampal neurons, J Cell Biol, vol.179, issue.7, pp.1511-1522, 2007. ,
Ganglioside GT1b as a complementary receptor component for Clostridium botulinum neurotoxins, Microb Pathog, vol.25, issue.2, pp.91-99, 1998. ,
Receptor binding enables botulinum neurotoxin B to sense low pH for translocation channel assembly, Cell Host Microbe, vol.10, issue.3, pp.237-247, 2011. ,
Tetanus toxin interaction with human erythrocytes. I. Properties of polysialoganglioside association with the cell surface, Biochim Biophys Acta, vol.812, issue.2, pp.523-531, 1985. ,
Gangliosides as high affinity receptors for tetanus neurotoxin, J Biol Chem, vol.284, issue.39, pp.26569-26577, 2009. ,
Localization of sites for 125I-labelled botulinum neurotoxin at murine neuromuscular junction and its binding to rat brain synaptosomes, Toxicon, vol.20, issue.1, pp.141-148, 1982. ,
Sialic acids in the brain: gangliosides and polysialic acid in nervous system development, stability, disease, and regeneration, Physiol Rev, vol.94, issue.2, pp.461-518, 2014. ,
Action-at-a-distance interactions enhance protein binding affinity, Protein Sci, vol.14, issue.5, pp.1363-1369, 2005. ,
Specific membrane binding of neurotoxin II can facilitate its delivery to acetylcholine receptor, Biophys J, vol.97, issue.7, pp.2089-2097, 2009. ,
The high-affinity binding of Clostridium botulinum type B neurotoxin to synaptotagmin II associated with gangliosides GT1b/GD1a, FEBS Lett, vol.378, issue.3, pp.253-257, 1996. ,
Synaptic proteins and SNARE complexes are localized in lipid rafts from rat brain synaptosomes, Biochem Biophys Res Commun, vol.329, issue.1, pp.117-124, 2005. ,
Hybrid In Silico/In Vitro Approaches for the Identification of Functional Cholesterol-Binding Domains in Membrane Proteins, Methods Mol Biol, vol.1583, pp.7-19, 2017. ,
CHARMM-GUI Membrane Builder for Complex Biological Membrane Simulations with Glycolipids and Lipoglycans, J Chem Theory Comput, vol.15, issue.1, pp.775-786, 2019. ,
CHARMM all-atom additive force field for sphingomyelin: elucidation of hydrogen bonding and of positive curvature, Biophys J, vol.107, issue.1, pp.134-145, 2014. ,
Additive empirical force field for hexopyranose monosaccharides, J Comput Chem, vol.29, issue.15, pp.2543-2564, 2008. ,
, One-way ANOVA followed by Bonferroni's post-hoc test was used for statistical analysis
, K 52 A p= 2.722 x 10 -5 (***); pSYT1 32-57 / pSYT9 p = 8.2 x 10 -8 (***)). (B) Effect of SYT1K 52 A mutation on BoNT/B binding in the absence of, pp.1-32
, BoNT/E (50 nM) binding (dashed curve) to pSYT1 was not distinguishable from the baseline. Data representative of 3 independent experiments. (C) Pull-down assays were carried out with recombinant GST-SYT1 and the K 52 A mutant immobilized on glutathione-Sepharose beads and incubated with 10 nM BoNT/B in presence or absence of GT1b. Analysis of pellets by protein staining confirmed identical amounts of GST-bait proteins. Pellets were analyzed by immunoblotting to reveal BoNT/B (150 kDa) that was partially reduced (heavy chain 100 kDa). The mutation decreased binding by 88 ± 2 % (mean ± SD n=4), GT1b. BoNT/B (30 nM) was injected for 2 min on pSYT peptides, vol.1, pp.41-52
, Proteoliposomes were captured on neutravidin sensorchips and BoNT/B (30 nM) was injected during 2 min. Results are representative of 4 independent experiments
, SYT1 K 52 A mutation induces a 93 ± 10% loss of BoNT/B binding taken at 60 s after the end of injection (n=4 independent experiments
, Mutation of the SYT1 juxtamembrane lysine (K 52 A) inhibits GT1b-dependent
, BoNT/B binding at the surface of PC12 cells. (A) Immunostaining of EGFP (green)
, and BoNT/B (red) in PC12?SYT1 cells expressing full length wild type synaptotagmin