, 1X Phosphate-buffered saline (PBS)

, 1X Phosphate-buffered saline with Ca 2+ and Mg 2+ (PBS(Ca/Mg))

, Fixation solution: 4% Paraformaldehyde (PFA)

, Permeabilization buffer for HeLa and RPE1 cells: 0.1% Triton X-100, 1 mg/ml BSA in PBS. Buffer should be passed through a sterile filter (0.2µm) to avoid cellular contamination for long-term storage

, Permeabilization buffer for IMCD cells: 0.3% Triton X-100, 10 mg/ml BSA, 4% donkey serum and 0.02% sodium azide in PBS (see Note 2)

, Blocking buffer for HeLa and RPE1 cells: 1 mg/ml BSA in PBS

, Blocking buffer for IMCD cells: 10 mg/ml BSA, 4% donkey serum and 0.02% sodium azide in PBS

, Quenching solution: 50 mM NH 4 Cl in PBS

, Mounting media for HeLa and RPE1 cells: PBS-glycerol mix (1:1) using the SlowFade Light Antifade Kit containing DAPI from Molecular Probes

, Mounting media for IMCD cells: Immu-Mount

, Microtubule depolymerizing solutions: Nocodazole (Sigma). 10µM for immediate depolymerization (1h). 1µM for cell-cycle synchronization (24h)

, Microtubule stabilizing solution: 10µM Taxol (Paclitaxel)

, Somatostatin-14 (Phoenix Pharmaceuticals)

, 3 Equipment: 1. Cells are grown in an incubator under standard growth conditions (37°C and 5% CO 2 )

, Cells are seeded on 12 mm glass coverslips

, For HeLa and RPE1 cells, coverslips are mounted on plain glass microscope slides (1mm, Pearl). For IMCD cells, coverslips are sandwiched between microscope cover glass, ThermoFisher Scientific) and a microscope slide

, 8-well µ-Slide, Ibidi

, 35 mm, high Glass Bottom, Ibidi. Neurons were plated on 35 mm glassbottomed (No. 1.5) dishes (MatTek) that had been coated with 40 µg

, Electroporation is performed in 0.4 cm gap Gene Pulser/MicroPulser cuvettes

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