Monitoring β-arrestin2 targeting to the centrosome, basal body and primary cilium by fluorescence microscopy: Centriolar and ciliary targeting of β-arrestin2

Anahi Mollà-Herman 1, 2, * Kathryn Davis 3 Kirk Mykytyn 3, * Alexandre Benmerah 4, *
* Corresponding author
1 Génétique et Biologie du Développement
2 CIRB - Centre interdisciplinaire de recherche en biologie
3 OSU - Ohio State University [Columbus]
4 IMAGINE - U1163 - Imagine - Institut des maladies génétiques
Abstract : Primary cilia (PC) are microtubule-based organelles that behave like a cellular antenna controlling key signaling pathways during development and tissue homeostasis. The ciliary membrane is highly enriched for G protein-coupled receptors (GPCRs), and PC are a crucial signaling compartment for this large receptor family. Downstream effectors of GPCR signaling are also present in cilia, and evidence obtained by our labs and others demonstrated that β-arrestin (βarr) family members are differentially recruited to PC and have investigated the role of GPCR activation in this process. In this chapter, we provide methods based on fluorescence microscopy on fixed or live cells suitable for investigating targeting and recruitment of βarrs at PC.
Keywords : cellular signaling arrestin basal body primary cilia centrosome
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Anahi Mollà-Herman, Kathryn Davis, Kirk Mykytyn, Alexandre Benmerah. Monitoring β-arrestin2 targeting to the centrosome, basal body and primary cilium by fluorescence microscopy: Centriolar and ciliary targeting of β-arrestin2. Methods Mol Biol, 1454, pp.271-289, 2019, ⟨10.1007/978-1-4939-9158-7_17⟩. ⟨inserm-02263790⟩

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