Monitoring β-arrestin2 targeting to the centrosome, basal body and primary cilium by fluorescence microscopy - Archive ouverte HAL Access content directly
Book Sections Year : 2019

Monitoring β-arrestin2 targeting to the centrosome, basal body and primary cilium by fluorescence microscopy

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Abstract

Primary cilia (PC) are microtubule-based organelles that behave like a cellular antenna controlling key signaling pathways during development and tissue homeostasis. The ciliary membrane is highly enriched for G protein-coupled receptors (GPCRs), and PC are a crucial signaling compartment for this large receptor family. Downstream effectors of GPCR signaling are also present in cilia, and evidence obtained by our labs and others demonstrated that β-arrestin (βarr) family members are differentially recruited to PC and have investigated the role of GPCR activation in this process. In this chapter, we provide methods based on fluorescence microscopy on fixed or live cells suitable for investigating targeting and recruitment of βarrs at PC.
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Dates and versions

inserm-02263790 , version 1 (05-08-2019)

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Anahi Mollà-Herman, Kathryn M Davis, Kirk Mykytyn, Alexandre Benmerah. Monitoring β-arrestin2 targeting to the centrosome, basal body and primary cilium by fluorescence microscopy: Centriolar and ciliary targeting of β-arrestin2. Methods Mol Biol, 1454, pp.271-289, 2019, ⟨10.1007/978-1-4939-9158-7_17⟩. ⟨inserm-02263790⟩
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