Diagnostic accuracy of a rapid RT-PCR assay for point-of-care detection of influenza A/B virus at emergency department admission: A prospective evaluation during the 2017/2018 influenza season

Maxime Maignan; Damien Viglino; Maud Hablot; Nicolas Masson; Anne Lebeugle; Roselyne Collomb Muret; Prudence Mabiala Makele; Valérie Guglielmetti; Patrice Morand; Julien Lupo; Virginie Forget; Caroline Landelle; Sylvie Larrat

doi:10.1371/journal.pone.0216308

Journal articles

Diagnostic accuracy of a rapid RT-PCR assay for point-of-care detection of influenza A/B virus at emergency department admission: A prospective evaluation during the 2017/2018 influenza season

Maxime Maignan ^{1, 2, *} Damien Viglino ^{1, 2} Maud Hablot ^{1, 2} Nicolas Masson ^{1, 2} Anne Lebeugle ^{1, 2} Roselyne Collomb Muret ^{1, 2} Prudence Mabiala Makele ^{1, 2} Valérie Guglielmetti ^{1, 2} Patrice Morand ^{3, 4} Julien Lupo ^{3, 4} Virginie Forget ^{5, 6} Caroline Landelle ^{6, 7} Sylvie Larrat ^{3, 4}

* Corresponding author

1 HP2 - Laboratoire Hypoxie et Physiopathologies cardiovasculaires et respiratoires [Grenoble]

2 Emergency department [Grenoble]

3 IBS - UMR 5075 - Institut de biologie structurale

4 Laboratoire de Virologie [Grenoble]

5 TIMC - Techniques de l'Ingénierie Médicale et de la Complexité - Informatique, Mathématiques et Applications, Grenoble - UMR 5525

6 Infection Control Unit [Grenoble]

7 TIMC-ThEMAS - Techniques pour l'Evaluation et la Modélisation des Actions de la Santé

TIMC - Techniques de l'Ingénierie Médicale et de la Complexité - Informatique, Mathématiques et Applications, Grenoble - UMR 5525

Abstract : STUDY OBJECTIVE: To investigate the performance of a rapid RT-PCR assay to detect influenza A/B at emergency department admission. METHODS: This single-center prospective study recruited adult patients attending the emergency department for influenza-like illness. Triage nurses performed nasopharyngeal swab samples and ran rapid RT-PCR assays using a dedicated device (cobas Liat, Roche Diagnostics, Meylan, France) located at triage. The same swab sample was also analyzed in the department of virology using conventional RT-PCR techniques. Patients were included 24 hours-a-day, 7 days-a-week. The primary outcome was the diagnostic accuracy of the rapid RT-PCR assay performed at triage. RESULTS: A total of 187 patients were included over 11 days in January 2018. Median age was 70 years (interquartile range 44 to 84) and 95 (51%) were male. Nine (5%) assays had to be repeated due to failure of the first assay. The sensitivity of the rapid RT-PCR assay performed at triage was 0.98 (95% confidence interval (CI): 0.91-1.00) and the specificity was 0.99 (95% CI: 0.94-1.00). A total of 92 (49%) assays were performed at night-time or during the weekend. The median time from patient entry to rapid RT-PCR assay results was 46 [interquartile range 36-55] minutes. CONCLUSION: Rapid RT-PCR assay performed by nurses at triage to detect influenza A/B is feasible and highly accurate.

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Journal articles

Domain :

Life Sciences [q-bio] / Human health and pathology / Infectious diseases

Life Sciences [q-bio] / Biochemistry, Molecular Biology

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Submitted on : Monday, July 29, 2019 - 10:41:34 AM
Last modification on : Friday, November 20, 2020 - 11:16:02 AM

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Diagnostic accuracy of a rapid RT-PCR assay for point-of-care detection of influenza A/B virus at emergency department admission: A prospective evaluation during the 2017/2018 influenza season

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Diagnostic accuracy of a rapid RT-PCR assay for point-of-care detection of influenza A/B virus at emergency department admission: A prospective evaluation during the 2017/2018 influenza season

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