Analysis and expression of a cloned pre-T cell receptor gene

Claude Saint-Ruf 1, 2, * Katarina Ungewiss 3 Markus Groettrup 3 Ludovica Bruno 3 Hans Fehling 3 Harald von Boehmer 3
* Corresponding author
1 Inserm U373 - Développement du système immunitaire
2 USPC - Université Sorbonne Paris Cité
3 BII - Basel Institute for Immunology
Abstract : The T cell antigen receptor (TCR) beta chain regulates early T cell development in the absence of the TCR alpha chain. The developmentally controlled gene described here encodes the pre-TCR alpha (pT alpha) chain, which covalently associates with TCR beta and with the CD3 proteins forms a pre-TCR complex that transduces signals in immature thymocytes. Unlike the lambda 5 pre-B cell receptor protein, the pT alpha chain is a type I transmembrane protein whose cytoplasmic tail contains two potential phosphorylation sites and a Src homology 3 (SH3)-domain binding sequence. Pre-TCR alpha transfection experiments indicated that surface expression of the pre-TCR is controlled by additional developmentally regulated proteins. Identification of the pT alpha gene represents an essential step in the structure-function analysis of the pre-TCR complex.
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Claude Saint-Ruf, Katarina Ungewiss, Markus Groettrup, Ludovica Bruno, Hans Fehling, et al.. Analysis and expression of a cloned pre-T cell receptor gene. Science, American Association for the Advancement of Science, 1994, 266 (5188), pp.1208-1212. ⟨10.1126/science.7973703⟩. ⟨inserm-02177273⟩

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