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Stress and Survival of Aging Escherichia coli rpoS Colonies

Abstract : In Escherichia coli, the expression of the RpoS regulon is known to be crucial for survival in liquid cultures during stationary phase. By measuring cell viability and by transcriptome analysis, here we show that rpoS cells as well as wild-type cells survive when they form colonies on solid media. E SCHERICHIA coli possess seven RNA polymerase While the decrease in the number of colony forming units (CFUs) obtained from planktonic cells between 1-factors, which compete for association with the core polymerase subunit, each coordinating the tran-day (D1) and 7 days (D7) in culture was 200-fold for rpoS mutant and 10-fold for wild-type strain (wt), there scription of a set of genes, which allows fine control of adaptation to different physiological conditions (Record was little difference in colonies between D1 and D7 for each strain as well as between the two strains (Table et al. 1996). In cells that approach stationary phase, the-factor 38 (RpoS) competes with the primary-factor 1a). Furthermore, the measurements of the cell viability in aging colonies, as well as of the colony area, show 70 (RpoD) for association with the core polymerase sub-unit. To date ‫001ف‬ genes have been reported to belong that the rpoS mutant had difficulties relative to wt during the first 24 hr (D1) but there was no difference between to the RpoS regulon whose induction results in physiological and morphological modifications that increase the two strains after 7 days (D7; Table 1b), indicating better survival of rpoS cells at D7 than at D1, and/or resistance to various stresses such as heat shock, cold shock, acid shock, oxidative stress, osmotic stress, and increased cell turnover. To identify the functions that might be responsible for fitness modifications of rpoS
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Claude Saint-Ruf, François Taddei, Ivan Matic. Stress and Survival of Aging Escherichia coli rpoS Colonies. Genetics, Genetics Society of America, 2004, 168 (1), pp.541-546. ⟨10.1534/genetics.104.028704⟩. ⟨inserm-02177257⟩



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