Reliable Detection of Dead Microbial Cells by Using Fluorescent Hydrazides

Abstract : We have developed a new method for accurate quantification of dead microbial cells. This technique employs the simultaneous use of fluorescent hydrazides and nucleic acid dyes. Fluorescent hydrazides allow detection of cells that cannot be detected with currently used high-affinity nucleic acid dyes. This is particularly important for nongrowing bacterial populations and for multicellular communities containing physiologically heterogeneous cell populations, such as colonies and biofilms.
Complete list of metadatas

Cited literature [13 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-02173973
Contributor : Claude Saint-Ruf <>
Submitted on : Thursday, July 4, 2019 - 6:52:31 PM
Last modification on : Sunday, July 7, 2019 - 1:24:34 AM

File

 Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed until : jamais

Please log in to resquest access to the document

Identifiers

Collections

Citation

Claude Saint-Ruf, Corinne Cordier, Jérome Megret, Ivan Matic. Reliable Detection of Dead Microbial Cells by Using Fluorescent Hydrazides. Applied and Environmental Microbiology, American Society for Microbiology, 2010, 76 (5), pp.1674-1678. ⟨10.1128/AEM.02125-09⟩. ⟨inserm-02173973⟩

Share

Metrics

Record views

73