Strong increase in the autofluorescence of cells signals struggle for survival

Jérémy Surre 1, 2 Claude Saint-Ruf 2, 3, 4 Valérie Collin 1 Sylvain Orenga 1 Mahendrasingh Ramjeet 1 Ivan Matic 5, 2, *
* Corresponding author
1 R&D Microbiologie - Unité de Microbiologie [La Balme Les Grottes, France]
2 U1001 - Robustesse et évolvabilité de la vie
3 IC UM3 (UMR 8104 / U1016) - Institut Cochin
4 USPC - Université Sorbonne Paris Cité
5 CNRS - Centre National de la Recherche Scientifique
Abstract : Prokaryotic and eukaryotic cells exhibit an intrinsic natural fluorescence due to the presence of fluorescent cellular structural components and metabolites. Therefore, cellular autofluorescence (AF) is expected to vary with the metabolic states of cells. We examined how exposure to the different stressors changes the AF of Escherichia coli cells. We observed that bactericidal treatments increased green cellular AF, and that de novo protein synthesis was required for the observed AF increase. Excitation and emission spectra and increased expression of the genes from the flavin biosynthesis pathway, strongly suggested that flavins are major contributors to the increased AF. An increased expression of genes encoding diverse flavoproteins which are involved in energy production and ROS detoxification, indicates a cellular strategy to cope with severe stresses. An observed increase in AF under stress is an evolutionary conserved phenomenon as it occurs not only in cells from different bacterial species, but also in yeast and human cells.
Document type :
Journal articles
Complete list of metadatas

Cited literature [47 references]  Display  Hide  Download
https://www.hal.inserm.fr/inserm-02173933
Contributor : Claude Saint-Ruf <>
Submitted on : Thursday, July 4, 2019 - 6:20:29 PM
Last modification on : Tuesday, July 9, 2019 - 11:49:40 AM

File

s41598-018-30623-2.pdf
Publisher files allowed on an open archive

Identifiers

Collections

UNIV-PARIS5 | USPC

Citation

Jérémy Surre, Claude Saint-Ruf, Valérie Collin, Sylvain Orenga, Mahendrasingh Ramjeet, et al.. Strong increase in the autofluorescence of cells signals struggle for survival. Scientific Reports, Nature Publishing Group, 2018, 8 (1), pp.12088. ⟨10.1038/s41598-018-30623-2⟩. ⟨inserm-02173933⟩

Export

BibTeX TEI DC DCterms EndNote

Share

Metrics

Record views

85

Files downloads

81

Contact

CCSD