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New Phosphorylation Sites of Rad51 by c-Met Modulates Presynaptic Filament Stability

Thomas Chabot 1 Alain Defontaine 1 Damien Marquis 1 Axelle Renodon-Cornière 2 Emmanuelle Courtois 1 Fabrice Fleury 1, * yvonnick Cheraud 1 
* Corresponding author
2 CRCINA-ÉQUIPE 13 - Nuclear Oncology
CRCINA - Centre de Recherche en Cancérologie et Immunologie Nantes-Angers
Abstract : Genomic instability through deregulation of DNA repair pathways can initiate cancer and subsequently result in resistance to chemo and radiotherapy. Understanding these biological mechanisms is therefore essential to overcome cancer. RAD51 is the central protein of the Homologous Recombination (HR) DNA repair pathway, which leads to faithful DNA repair of DSBs. The recombinase activity of RAD51 requires nucleofilament formation and is regulated by post-translational modifications such as phosphorylation. In the last decade, studies have suggested the existence of a relationship between receptor tyrosine kinases (RTK) and Homologous Recombination DNA repair. Among these RTK the c-MET receptor is often overexpressed or constitutively activated in many cancer types and its inhibition induces the decrease of HR. In this study, we show for the first time that c-MET is able to phosphorylate the RAD51 protein. We demonstrate in vitro that c-MET phosphorylates four tyrosine residues localized mainly in the subunit-subunit interface of RAD51. Whereas these post-translational modifications do not affect the presynaptic filament formation, they strengthen its stability against the inhibitor effect of the BRC peptide obtained from BRCA2. Taken together, these results confirm the role of these modifications in the regulation of the BRCA2-RAD51 interaction and underline the importance of c-MET in DNA damage response.
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Submitted on : Friday, June 28, 2019 - 1:58:56 PM
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Thomas Chabot, Alain Defontaine, Damien Marquis, Axelle Renodon-Cornière, Emmanuelle Courtois, et al.. New Phosphorylation Sites of Rad51 by c-Met Modulates Presynaptic Filament Stability. Cancers, MDPI, 2019, 11 (3), pp.413. ⟨10.3390/cancers11030413⟩. ⟨inserm-02168094⟩



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