Soluble HLA-G in IVF/ICSI embryo culture supernatants does not always predict implantation success: a multicentre study.

Julie Tabiasco 1 Sophie Perrier D'hauterive 2 Fabienne Thonon 2 Jean Parinaud 3 Roger Léandri 3 Jean-Michel Foidart 2 Gérard Chaouat 4 Carine Munaut 2 Raoul Lombroso 5 Jacqueline Selva 5 Marianne Bergère 5 Ibrahim Hammoud 5 Noemi Kozma 1 Maryse Aguerre-Girr 1 Anna Swales 6 Ian Sargent 6 Philippe Le Bouteiller 1, 3, * Nathalie Lédée 5, *
* Corresponding author
1 CPTP - Centre de Physiopathologie Toulouse Purpan (ex IFR 30 et IFR 150)
2 Département de Gynécologie et Obstétrique, CPMA
3 Laboratoire de Biologie de la Reproduction [CHU Toulouse]
4 Endocrinologie et Génétique de la Reproduction et du Développement
5 Médecine et Biologie de la Reproduction
6 Oxford Fertility Unit
Abstract : Several reports have described an association between the presence of soluble human leukocyte antigen G (sHLA-G) in human embryo culture supernatants (ES) and implantation success. However, not all studies agree with these findings. To further document this debate, a multicentre blinded study was performed to investigate, on a large number of IVF ES and ICSI ES, whether sHLA-G is a useful criterion for embryo selection before transfer. A total of 1405 ES from 355 patients were collected from three assisted reproductive technique (ART) centres and evaluated for their sHLA-G content in a single laboratory, using a chemiluminescence enzyme-linked immunosorbent assay. In only one centre was a significant association between sHLA-G-positive ES and successful implantation established (P = 0.0379), whereas no such association was observed in the other centres. It was found that the percentages and concentrations of sHLA-G-positive ES varied between centres, depending on culture media and ART conditions. The percentage of sHLA-G-positive ES was significantly higher in IVF ES than ICSI ES (P < 0.001 and P < 0.01 for two centres). These data demonstrate that substantial variations of sHLA-G content in ES occur between different ART centres, highlighting the influence of several technical parameters that differ from one centre to another.
Keywords : IVF ICSI soluble HLA-G embryo transfer
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  • HAL Id : inserm-02167997, version 1
  • PUBMED : 19298737

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Julie Tabiasco, Sophie Perrier D'hauterive, Fabienne Thonon, Jean Parinaud, Roger Léandri, et al.. Soluble HLA-G in IVF/ICSI embryo culture supernatants does not always predict implantation success: a multicentre study.. Reproductive BioMedicine Online, Elsevier, 2009, 18 (3), pp.374 - 381. ⟨inserm-02167997⟩

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