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Journal articles
Soluble HLA-G in IVF/ICSI embryo culture supernatants does not always predict implantation success: a multicentre study.
Julie Tabiasco
1
Sophie Perrier D'hauterive
2
Fabienne Thonon
2
Jean Parinaud
3
Roger Léandri
3
Jean-Michel Foidart
2
Gérard Chaouat
4
Carine Munaut
2
Raoul Lombroso
5
Jacqueline Selva
5
Marianne Bergère
5
Ibrahim Hammoud
5
Noemi Kozma
1
Maryse Aguerre-Girr
1
Anna Swales
6
Ian Sargent
6
Philippe Le Bouteiller
1, 3, *
Nathalie Lédée
5, *
*
Corresponding author
Julie Tabiasco 1
Author
Sophie Perrier D'hauterive 2
Author
Fabienne Thonon 2
Author
Jean Parinaud 3
Author
Raoul Lombroso 5
Author
Jacqueline Selva 5
Author
Marianne Bergère 5
Author
Ibrahim Hammoud 5
Author
Philippe Le Bouteiller 1, 3
Correspondent author
Nathalie Lédée 5
Correspondent author
1
CPTP - Centre de Physiopathologie Toulouse Purpan ex IFR 30 et IFR 150 (Hôpital de Purpan Place du Docteur Baylac 31024 TOULOUSE CEDEX 3 - France)
- UT3 - Université Toulouse III - Paul Sabatier (118 route de Narbonne - 31062 Toulouse - France)
- Université Fédérale Toulouse Midi-Pyrénées (41 Allée Jules Guesde, 31000 Toulouse - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U1043 (101, rue de Tolbiac, 75013 Paris - France)
- CNRS - Centre National de la Recherche Scientifique : UMR5282 (France)
2
Département de Gynécologie et Obstétrique, CPMA (Liège - Belgium)
- CHR Citadelle (Belgium)
- Université de Liège (Place du 20-Août, 7 4000 Liège - Belgium)
3
Laboratoire de Biologie de la Reproduction [CHU Toulouse] (France)
- CHU Toulouse [Toulouse] (Hôtel-Dieu Saint Jacques 2, rue Viguerie TSA 80035 31059 Toulouse cedex 9 - France)
- Hôpital Paule de Viguier (France)
4
Endocrinologie et Génétique de la Reproduction et du Développement (Centre de Recherche Inserm 32, Rue Des Carnets 92140 Clamart - France)
- UP11 - Université Paris-Sud - Paris 11 (Bâtiment 300 - 91405 Orsay cedex - France)
- IFR13 (France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U782 (101, rue de Tolbiac, 75013 Paris - France)
5
BREED - Biologie de la Reproduction, Environnement, Epigénétique & Développement Unité de recherche (INRA Domaine de Vilvert
78352 Jouy en Josas - France)
- Université Paris-Saclay (Espace Technologique, Bat. Discovery - RD 128 - 2e ét., 91190 Saint-Aubin - France)
- UVSQ - Université de Versailles Saint-Quentin-en-Yvelines : UMR1198 (55 avenue de Paris - 78035 Versailles cedex - France)
- ENVA - École nationale vétérinaire d'Alfort (7 avenue du Général de Gaulle - 94700 Maisons Alfort - France)
- INRAE - Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement : UMR 1198 (France)
6
Oxford Fertility Unit (Oxford - United Kingdom)
- John Radcliffe Hospital [Oxford University Hospital] (Headley Way, Headington, Oxford OX3 9DU - United Kingdom)
Abstract : Several reports have described an association between the presence of soluble human leukocyte antigen G (sHLA-G) in human embryo culture supernatants (ES) and implantation success. However, not all studies agree with these findings. To further document this debate, a multicentre blinded study was performed to investigate, on a large number of IVF ES and ICSI ES, whether sHLA-G is a useful criterion for embryo selection before transfer. A total of 1405 ES from 355 patients were collected from three assisted reproductive technique (ART) centres and evaluated for their sHLA-G content in a single laboratory, using a chemiluminescence enzyme-linked immunosorbent assay. In only one centre was a significant association between sHLA-G-positive ES and successful implantation established (P = 0.0379), whereas no such association was observed in the other centres. It was found that the percentages and concentrations of sHLA-G-positive ES varied between centres, depending on culture media and ART conditions. The percentage of sHLA-G-positive ES was significantly higher in IVF ES than ICSI ES (P < 0.001 and P < 0.01 for two centres). These data demonstrate that substantial variations of sHLA-G content in ES occur between different ART centres, highlighting the influence of several technical parameters that differ from one centre to another.
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Journal articles
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https://www.hal.inserm.fr/inserm-02167997
Contributor : Tabiasco Julie <>
Submitted on : Friday, June 28, 2019 - 12:50:58 PM
Last modification on : Wednesday, September 16, 2020 - 5:02:26 PM
Contributor : Tabiasco Julie <>
Submitted on : Friday, June 28, 2019 - 12:50:58 PM
Last modification on : Wednesday, September 16, 2020 - 5:02:26 PM
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Identifiers
- HAL Id : inserm-02167997, version 1
- PUBMED : 19298737
Collections
UNIV-TLSE3 | UVSQ | CNRS | INRAE | BREED
Citation
Julie Tabiasco, Sophie Perrier D'hauterive, Fabienne Thonon, Jean Parinaud, Roger Léandri, et al.. Soluble HLA-G in IVF/ICSI embryo culture supernatants does not always predict implantation success: a multicentre study.. Reproductive BioMedicine Online, Elsevier, 2009, 18 (3), pp.374 - 381. ⟨inserm-02167997⟩
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