Characterization of Antigen-Specific B Cells Using Nominal Antigen-Coated Flow-Beads

Abstract : In order to characterize the reactivity of B cells against nominal antigens, a method based on the coupling of antigens onto the surface of fluorescent core polystyrene beads was developed. We first demonstrate that murine B cells with a human MOG-specific BCR are able to interact with MOG-coated beads and do not recognize beads coated with human albumin or pp65. B cells purified from human healthy volunteer blood or immunized individuals were tested for their ability to interact with various nominal antigens, including viral, vaccine, self and alloantigens, chosen for their usefulness in studying a variety of pathological processes. A substantial amount of B cells binding self-antigen MOG-coated beads can be detected in normal blood. Furthermore, greater frequencies of B cell against anti-Tetanic Toxin or anti-EBNA1 were observed in primed individuals. This method can reveal increased frequencies of anti-HLA committed B cells in patients with circulating anti-HLA antibodies compared to unsensitized patients and normal individuals. Of interest, those specific CD19 cells were preferentially identified within CD27 2 IgD + (i-e naïve) subset. These observations suggest that a broad range of medical situations could benefit from a tool that allows the detection, the quantification and the characterization of antigen-specific blood B cells.
Document type :
Journal articles
Complete list of metadatas

Cited literature [34 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-02165805
Contributor : Ana Paula Dutra Azevedo <>
Submitted on : Wednesday, June 26, 2019 - 11:10:30 AM
Last modification on : Thursday, July 11, 2019 - 2:10:09 PM

File

pone.0084273.pdf
Publisher files allowed on an open archive

Identifiers

Collections

Citation

Nicolas Degauque, Annie Ngono, Ahmed Akl, Maud Lepetit, Romain Crochette, et al.. Characterization of Antigen-Specific B Cells Using Nominal Antigen-Coated Flow-Beads. PLoS ONE, Public Library of Science, 2013, 8 (12), pp.e84273. ⟨10.1371/journal.pone.0084273⟩. ⟨inserm-02165805⟩

Share

Metrics

Record views

23

Files downloads

42