Carbon monoxide-treated dendritic cells decrease β1-integrin induction on CD8 + T cells and protect from type 1 diabetes

Abstract : Carbon monoxide (CO) treatment improves pathogenic outcome of autoimmune diseases by promoting tolerance. However, the mechanism behind this protective tolerance is not yet defined. Here, we show in a transgenic mouse model for autoimmune diabetes that ex vivo gaseous CO (gCO)-treated DCs loaded with pancreatic β-cell peptides protect mice from disease. This protection is peptide-restricted, independent of IL-10 secretion by DCs and of CD4 + T cells. Although no differences were observed in autoreactive CD8 + T-cell function from gCO-treated versus untreated DC-immunized groups, gCO-treated DCs strongly inhibited accumulation of autoreactive CD8 + T cells in the pancreas. Interestingly , induction of β1-integrin was curtailed when CD8 + T cells were primed with gCO-treated DCs, and the capacity of these CD8 + T cells to lyse isolated islet was dramatically impaired. Thus, immunotherapy using CO-treated DCs appears to be an original strategy to control autoimmune disease.
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Thomas Simon, Sylvie Pogu, Virginie Tardif, Kevin Rigaud, Severine Rémy, et al.. Carbon monoxide-treated dendritic cells decrease β1-integrin induction on CD8 + T cells and protect from type 1 diabetes. European Journal of Immunology, Wiley-VCH Verlag, 2013, 43 (1), pp.209-218. ⟨10.1002/eji.201242684⟩. ⟨inserm-02164776⟩

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