Urine-derived cells provide a readily accessible cell type for feeder-free mRNA reprogramming

A. Gaignerie 1 N. Lefort 2 M. Rousselle 1 V. Forest-Choquet 3 L. Flippe 4, 5, 6 V Francois-Campion 4, 5, 6 A. Girardeau 3 A. Caillaud 3 C. Chariau 1 Q. Francheteau 1 A. Derevier 1 F. Chaubron 7 S. Knöbel 8 N. Gaborit 3 K. Si-Tayeb 3 L. David 4, 1, 5, 6, *
* Corresponding author
1 CHU Nantes/INSERM/CNRS-IFR Santé François Bonamy - Plate-forme Cellules souches pluripotentes induites - iPSC [Nantes]
SFR RSFB - Structure Fédérative de Recherche en Santé François Bonamy, CHU Nantes - Centre hospitalier universitaire de Nantes, INSERM - Institut National de la Santé et de la Recherche Médicale, CNRS - Centre National de la Recherche Scientifique, UN - Université de Nantes
2 IPS Platform [Paris]
IMAGINE - U1163 - Imagine - Institut des maladies génétiques
Abstract : Over a decade after their discovery, induced pluripotent stem cells (iPSCs) have become a major biological model. The iPSC technology allows generation of pluripotent stem cells from somatic cells bearing any genomic background. The challenge ahead of us is to translate human iPSCs (hiPSCs) protocols into clinical treatment. To do so, we need to improve the quality of hiPSCs produced. In this study we report the reprogramming of multiple patient urine-derived cell lines with mRNA reprogramming, which, to date, is one of the fastest and most faithful reprogramming method. We show that mRNA reprogramming efficiently generates hiPSCs from urine-derived cells. Moreover, we were able to generate feeder-free bulk hiPSCs lines that did not display genomic abnormalities. Altogether, this reprogramming method will contribute to accelerating the translation of hiPSCs to therapeutic applications.
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A. Gaignerie, N. Lefort, M. Rousselle, V. Forest-Choquet, L. Flippe, et al.. Urine-derived cells provide a readily accessible cell type for feeder-free mRNA reprogramming. Scientific Reports, Nature Publishing Group, 2018, 8 (1), pp.14363. ⟨10.1038/s41598-018-32645-2⟩. ⟨inserm-02161205⟩

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