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Journal articles
The RBM14/CoAA-interacting, long intergenic non-coding RNA Paral1 regulates adipogenesis and coactivates the nuclear receptor PPARγ
François Firmin
1
Frédérik Oger
1
Celine Gheeraert
1
Julie Dubois-Chevalier
1
Anne-Sophie Vercoutter-Edouart
2, 3
Fawaz Alzaid
4
Claire Mazuy
1
Hélène Dehondt
1
Jérémy Alexandre
1
Bruno Derudas
1
Quentin Dhalluin
1
Maheul Ploton
1
Alexandre Berthier
1
Eloise Woitrain
1
Tony Lefebvre
2
Nicolas Venteclef
4
François Pattou
5
Bart Staels
1
Jérôme Eeckhoute
1
Philippe Lefebvre
1, *
*
Corresponding author
François Firmin 1
Author
Frédérik Oger 1
Author
Celine Gheeraert 1
Author
Julie Dubois Dubois-Chevalier 1
Author
Anne-Sophie Vercoutter-Edouart 2, 3
Author IdHAL : anne-sophie-vercoutter-edouart ORCID : https://orcid.org/0000-0002-3243-7122
Fawaz Alzaid 4
Author
Claire Mazuy 1
Author
Hélène Dehondt 1
Author
Alexandre Berthier 1
Author
Eloise Woitrain 1
Author
Tony Lefebvre 2
Author
Nicolas Venteclef 4
Author
François Pattou 5
Author
Bart Staels 1
Author IdHAL : bart-staels ResearcherId : http://www.researcherid.com/rid/N-9497-2016 ORCID : https://orcid.org/0000-0002-3784-1503
Jérôme Eeckhoute 1
Author IdHAL : jerome-eeckhoute ORCID : https://orcid.org/0000-0002-7222-9264
Philippe Lefebvre 1
Correspondent author
1
RNMCD - Récepteurs nucléaires, maladies cardiovasculaires et diabète - U 1011 (1 rue du Prof Calmette
- 59019 Lille Cedex - France)
- Institut Pasteur de Lille (1 Rue du Professeur Calmette - Lille Cedex - 59019 - France)
- RIIP - Réseau International des Instituts Pasteur (25, rue du Dr Roux 75724 Paris Cedex 15 - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U1011 (101, rue de Tolbiac, 75013 Paris - France)
- Université de Lille : U1011 (Lille - France)
- CHRU Lille - Centre Hospitalier Régional Universitaire [Lille] (2, avenue Oscar Lambret - 59037 Lille Cedex - France)
2
UGSF - Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (Université de Lille Sciences et technologies - UMR8576 CNRS Cité Scientifique - Bâtiment C9 F-59655 Villeneuve d’Ascq Cedex - France)
- INRA - Institut National de la Recherche Agronomique : USC1409 (France)
- Université de Lille (Lille - France)
- CNRS - Centre National de la Recherche Scientifique : UMR8576 (France)
3
FRABio - Biochimie Structurale et Fonctionnelle des Assemblages Biomoléculaires - CNRS FR3688 (Villeneuve d’Ascq F-59655.
Biochimie Structurale et Fonctionnelle des Assemblages Biomoléculaires
- France)
- Université de Lille (Lille - France)
- CNRS - Centre National de la Recherche Scientifique : FR3688 (France)
4
CRC (UMR_S_1138 / U1138) - Centre de Recherche des Cordeliers (15 rue de l'Ecole de Médecine - Bâtiment B -
75270 Paris Cedex 06 - France)
- EPHE - École pratique des hautes études (4-14 Rue Ferrus, 75014 Paris - France)
- PSL - Université Paris sciences et lettres (60 rue Mazarine 75006 Paris - France)
- UPD7 - Université Paris Diderot - Paris 7 : UMR_S_1138 (5 rue Thomas-Mann - 75205 Paris cedex 13 - France)
- UPD5 - Université Paris Descartes - Paris 5 : UMR_S_1138 (12, rue de l'École de Médecine - 75270 Paris cedex 06 - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U1138 (101, rue de Tolbiac, 75013 Paris - France)
- SU - Sorbonne Université (21 rue de l’École de médecine - 75006 Paris - France)
5
RTD - Recherche translationnelle sur le diabète - U 1190 (- INSERM U1190
- Recherche translationnelle sur le diabète (RTD)
(Translational research for Diabetes - TRD)
- faculté de médecine pôle Recherche
- 3eme étage ouest
- Place de Verdun
- 59045 Lille Cedex - France)
- Institut Pasteur de Lille (1 Rue du Professeur Calmette - Lille Cedex - 59019 - France)
- RIIP - Réseau International des Instituts Pasteur (25, rue du Dr Roux 75724 Paris Cedex 15 - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U1190 (101, rue de Tolbiac, 75013 Paris - France)
- Université de Lille : U859 (Lille - France)
- CHRU Lille - Centre Hospitalier Régional Universitaire [Lille] : U859 (2, avenue Oscar Lambret - 59037 Lille Cedex - France)
Abstract : Adipocyte differentiation and function relies on a network of transcription factors, which is disrupted in obesity-associated low grade, chronic inflammation leading to adipose tissue dysfunction. In this context, there is a need for a thorough understanding of the transcriptional regulatory network involved in adipose tissue pathophysiology. Recent advances in the functional annotation of the genome has highlighted the role of non-coding RNAs in cellular differentiation processes in coordination with transcription factors. Using an unbiased genome-wide approach, we identified and characterized a novel long intergenic non-coding RNA (lincRNA) strongly induced during adipocyte differentiation. This lincRNA favors adipocyte differentiation and coactivates the master adipogenic regulator peroxisome proliferator-activated receptor gamma (PPARγ) through interaction with the paraspeckle component and hnRNP-like RNA binding protein 14 (RBM14/NCoAA), and was therefore called PPARγ-activator RBM14-associated lncRNA (Paral1). Paral1 expression is restricted to adipocytes and decreased in humans with increasing body mass index. A decreased expression was also observed in diet-induced or genetic mouse models of obesity and this down-regulation was mimicked in vitro by TNF treatment. In conclusion, we have identified a novel component of the adipogenic transcriptional regulatory network defining the lincRNA Paral1 as an obesity-sensitive regulator of adipocyte differentiation and function.
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Journal articles
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https://www.hal.inserm.fr/inserm-02159068
Contributor : Marie-Hélène Derudas Connect in order to contact the contributor
Submitted on : Tuesday, June 18, 2019 - 2:52:16 PM
Last modification on : Tuesday, October 19, 2021 - 11:33:27 AM
Contributor : Marie-Hélène Derudas Connect in order to contact the contributor
Submitted on : Tuesday, June 18, 2019 - 2:52:16 PM
Last modification on : Tuesday, October 19, 2021 - 11:33:27 AM
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Firmin Scientific Rep.pdf
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Identifiers
- HAL Id : inserm-02159068, version 1
- DOI : 10.1038/s41598-017-14570-y
- PUBMED : 29075020
Collections
INSERM | INRA | RIIP | RIIP_LILLE | CNRS | UNIV-PARIS5 | UNIV-LILLE | UNIV-PARIS7 | CORDELIERS | EPHE | USPC | SORBONNE-UNIVERSITE | SU-SCIENCES | INRAE | UNIV-PARIS | UP-SANTE | PSL | RNMCD | SU-TI
Citation
François Firmin, Frédérik Oger, Celine Gheeraert, Julie Dubois-Chevalier, Anne-Sophie Vercoutter-Edouart, et al.. The RBM14/CoAA-interacting, long intergenic non-coding RNA Paral1 regulates adipogenesis and coactivates the nuclear receptor PPARγ. Scientific Reports, Nature Publishing Group, 2017, 7 (1), pp.14087. ⟨10.1038/s41598-017-14570-y⟩. ⟨inserm-02159068⟩
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