The RBM14/CoAA-interacting, long intergenic non-coding RNA Paral1 regulates adipogenesis and coactivates the nuclear receptor PPARγ

François Firmin; Frédérik Oger; Celine Gheeraert; Julie Dubois-Chevalier; Anne-Sophie Vercoutter-Edouart; Fawaz Alzaid; Claire Mazuy; Hélène Dehondt; Jérémy Alexandre; Bruno Derudas; Quentin Dhalluin; Maheul Ploton; Alexandre Berthier; Eloise Woitrain; Tony Lefebvre; Nicolas Venteclef; François Pattou; Bart Staels; Jérôme Eeckhoute; Philippe Lefebvre

doi:10.1038/s41598-017-14570-y

Journal articles

The RBM14/CoAA-interacting, long intergenic non-coding RNA Paral1 regulates adipogenesis and coactivates the nuclear receptor PPARγ

François Firmin ¹ Frédérik Oger ¹ Celine Gheeraert ¹ Julie Dubois-Chevalier ¹ Anne-Sophie Vercoutter-Edouart ^{2, 3} Fawaz Alzaid ⁴ Claire Mazuy ¹ Hélène Dehondt ¹ Jérémy Alexandre ¹ Bruno Derudas ¹ Quentin Dhalluin ¹ Maheul Ploton ¹ Alexandre Berthier ¹ Eloise Woitrain ¹ Tony Lefebvre ² Nicolas Venteclef ⁴ François Pattou ⁵ Bart Staels ¹ Jérôme Eeckhoute ¹ Philippe Lefebvre ^{1, *}

* Corresponding author

1 RNMCD - Récepteurs nucléaires, maladies cardiovasculaires et diabète - U 1011

2 UGSF - Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576

3 FRABio - Biochimie Structurale et Fonctionnelle des Assemblages Biomoléculaires - CNRS FR3688

4 CRC (UMR_S_1138 / U1138) - Centre de Recherche des Cordeliers

5 RTD - Recherche translationnelle sur le diabète - U 1190

Abstract : Adipocyte differentiation and function relies on a network of transcription factors, which is disrupted in obesity-associated low grade, chronic inflammation leading to adipose tissue dysfunction. In this context, there is a need for a thorough understanding of the transcriptional regulatory network involved in adipose tissue pathophysiology. Recent advances in the functional annotation of the genome has highlighted the role of non-coding RNAs in cellular differentiation processes in coordination with transcription factors. Using an unbiased genome-wide approach, we identified and characterized a novel long intergenic non-coding RNA (lincRNA) strongly induced during adipocyte differentiation. This lincRNA favors adipocyte differentiation and coactivates the master adipogenic regulator peroxisome proliferator-activated receptor gamma (PPARγ) through interaction with the paraspeckle component and hnRNP-like RNA binding protein 14 (RBM14/NCoAA), and was therefore called PPARγ-activator RBM14-associated lncRNA (Paral1). Paral1 expression is restricted to adipocytes and decreased in humans with increasing body mass index. A decreased expression was also observed in diet-induced or genetic mouse models of obesity and this down-regulation was mimicked in vitro by TNF treatment. In conclusion, we have identified a novel component of the adipogenic transcriptional regulatory network defining the lincRNA Paral1 as an obesity-sensitive regulator of adipocyte differentiation and function.

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Life Sciences [q-bio]

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https://www.hal.inserm.fr/inserm-02159068
Contributor : Marie-Hélène Derudas <>
Submitted on : Tuesday, June 18, 2019 - 2:52:16 PM
Last modification on : Monday, February 22, 2021 - 4:12:01 PM

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