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Journal articles
Hypothermic pulsatile perfusion of human pancreas: Preliminary technical feasibility study based on histology
Abstract : Background: There are currently two approaches to hypothermic preservation for most solid organs: static or dynamic. Cold storage is the main method used for static storage (SS), while hypothermic pulsatile perfusion (HPP) and other machine perfusion-based methods, such as normothermic machine perfusion and oxygen persufflation, are the methods used for dynamic preservation. HPP is currently approved for kidney trans-plantation.
Methods: We evaluated, for the first time, the feasibility of HPP on 11 human pancreases contraindicated for clinical transplantation because of advanced age and/or history of severe alcoholism and/or abnormal laboratory tests. Two pancreases were used as SS controls, pancreas splitting was performed on 2 other pancreases for SS and HPP and 7 pancreases were tested for HPP. HPP preservation lasted 24 h at 25 mmHg. Resistance index was continuously monitored and pancreas and duodenum histology was evaluated every 6 h.
Results: The main finding was the complete absence of edema of the pancreas and duodenum at all time-points during HPP. Insulin, glucagon and somatostatin staining was normal. Resistance index decreased during the first 12 h and remained stable thereafter.
Conclusion: 24 h hypothermic pulsatile perfusion of marginal human pancreas-duodenum organs was feasible with no deleterious parenchymal effect. These observations encourage us to further develop this technique and evaluate the safety of HPP after clinical transplantation.
Cited literature [31 references]
https://www.hal.inserm.fr/inserm-02157509
Contributor : Sylvie Le Bihan Connect in order to contact the contributor
Submitted on : Monday, June 17, 2019 - 8:31:34 AM
Last modification on : Wednesday, June 26, 2019 - 1:51:55 PM
Contributor : Sylvie Le Bihan Connect in order to contact the contributor
Submitted on : Monday, June 17, 2019 - 8:31:34 AM
Last modification on : Wednesday, June 26, 2019 - 1:51:55 PM
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