Morphokinetic parameters in chromosomal translocation carriers undergoing preimplantation genetic testing

Abstract : RESEARCH QUESTION: Can embryo morphokinetic parameters help identify unbalanced embryos in translocation carriers? DESIGN: This retrospective study was conducted in 67 translocation carriers undergoing 105 preimplantation genetic testing cycles for chromosomal structural rearrangements (PGT-SR) without aneuploidy screening (PGT-A). Using time-lapse imaging analysis, morphokinetic parameters of balanced and unbalanced embryos were compared, as well as the frequency of abnormal cellular events. The performance of a previously published prediction model of aneuploidy was also tested in this population. RESULTS: Significant differences were observed between balanced and unbalanced embryos for some morphokinetic parameters: t5 (P = 0.0067), t9+ (P = 0.0077), cc2 (P = 0.0144), s2 (P = 0.0003) and t5-t2 (P = 0.0028). Also, multinucleation at the two- or four-cell stages, abnormal division and cell exclusion at the morula stage were significantly (all P < 0.05) more frequent in unbalanced than in balanced embryos. None, however, could accurately predict embryo chromosomal status. A previously published morphokinetic prediction model for embryo aneuploidy did not adequately classify balanced and unbalanced embryos. CONCLUSIONS: No significant morphokinetic predictor of chromosomal status could be found. Time-lapse should not be used as a diagnostic tool for chromosomal status in translocation carriers.
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Jenna Lammers, Arnaud Reignier, Carole Splingart, Kamran Moradkhani, Paul Barrière, et al.. Morphokinetic parameters in chromosomal translocation carriers undergoing preimplantation genetic testing. Reproductive BioMedicine Online, Elsevier, 2018, 38. ⟨inserm-02156313⟩

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