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Journal articles
Heme Oxygenase-1 Modulates Human Respiratory Syncytial Virus Replication and Lung Pathogenesis during Infection
Janyra Espinoza
1
Miguel León
1
Pablo Céspedes
1
Roberto Gomez
1
Gisela Canedo-Marroquín
1
Sebastián Riquelme
1
Francisco Salazar-Echegarai
1
Phillipe Blancou
2
Thomas Simon
2
Ignacio Anegon
2
Margarita Lay
3
Pablo Gonzalez
1
Claudia Riedel
4
Susan Bueno
1
Alexis Kalergis
2, 1, 5, *
*
Corresponding author
Janyra A Espinoza 1
Author
Miguel A León 1
Author
Pablo F Céspedes 1
Author
Roberto S. Gomez 1
Author
Gisela Canedo-Marroquín 1
Author
Sebastián A Riquelme 1
Author
Francisco J Salazar-Echegarai 1
Author
Phillipe Blancou 2
Author
1
Facultad de Ciencias Biologicas - Departamento de Microbiologıa y Genetica Molecular [Santiago, Chile] (Chile)
- UC - Pontificia Universidad Católica de Chile (Casilla 306, Santiago 22 - Chile)
2
U1064 Inserm - CRTI - Centre de Recherche en Transplantation et Immunologie (CHU Nantes - Hôtel Dieu - 30 Bd Jean Monnet 44093 Nantes Cedex 01. - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U1064 (101, rue de Tolbiac, 75013 Paris - France)
- UFR MEDECINE - Université de Nantes - UFR de Médecine et des Techniques Médicales (1 rue Gaston Veil BP 53508 44035 NANTES Cedex 1 - France)
- UN - Université de Nantes (1, quai de Tourville - BP 13522 - 44035 Nantes cedex 1 - France)
5
Facultad de Medicina - Departamento de Endocrinología [Santiago, Chile] (Chile)
- UC - Pontificia Universidad Católica de Chile (Casilla 306, Santiago 22 - Chile)
Abstract : Human respiratory syncytial virus (hRSV) is the leading cause of severe lower respiratory tract infections in children. The development of novel prophylactic and therapeutic antiviral drugs against hRSV is imperative to control the burden of disease in the susceptiblepopulation.Inthisstudy,weexaminedtheeffectsofinducingtheactivityofthehostenzymehemeoxygenase-1(HO-1)on hRSV replication and pathogenesis on lung inflammation induced by this virus. Our results show that after hRSV infection, HO-1 induction with metalloporphyrin cobalt protoporphyrin IX significantly reduces the loss of body weight due to hRSV-induced disease. Further, HO-1 induction also decreased viral replication and lung inflammation, as evidenced by a reduced neutrophil infiltration into the airways, with diminished cytokine and chemokine production and reduced T cell function. Concomitantly, upon cobalt protoporphyrin IX treatment, there is a significant upregulation in the production of IFN-a/b mRNAs in the lungs. Furthermore, similar antiviral and protective effects occur by inducing the expression of human HO-1 in MHC class II+ cells in transgenic mice. Finally, in vitro data suggest that HO-1 induction can modulate the susceptibility of cells, especially the airway epithelial cells, to hRSV infection.
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https://www.hal.inserm.fr/inserm-02154790
Contributor : Sylvie Le Bihan <>
Submitted on : Thursday, June 13, 2019 - 8:42:09 AM
Last modification on : Tuesday, August 13, 2019 - 2:30:12 PM
Contributor : Sylvie Le Bihan <>
Submitted on : Thursday, June 13, 2019 - 8:42:09 AM
Last modification on : Tuesday, August 13, 2019 - 2:30:12 PM
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- HAL Id : inserm-02154790, version 1
- DOI : 10.4049/jimmunol.1601414
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Citation
Janyra Espinoza, Miguel León, Pablo Céspedes, Roberto Gomez, Gisela Canedo-Marroquín, et al.. Heme Oxygenase-1 Modulates Human Respiratory Syncytial Virus Replication and Lung Pathogenesis during Infection. Journal of Immunology, Publisher : Baltimore : Williams & Wilkins, c1950-. Latest Publisher : Bethesda, MD : American Association of Immunologists, 2017, 199 (1), pp.212-223. ⟨10.4049/jimmunol.1601414⟩. ⟨inserm-02154790⟩