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Book Sections Year : 2016

Genome Editing in Rats Using TALE Nucleases

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Anne de Cian


The rat is an important animal model to understand gene function and model human diseases. Since recent years, the development of gene-specific nucleases has become important for generating new rat models of human diseases, to analyze the role of genes and to generate human antibodies. Transcription activator-like (TALE) nucleases efficiently create gene-specific knockout rats and lead to the possibility of gene targeting by homology-directed recombination (HDR) and generating knock-in rats. We describe a detailed protocol for generating knockout and knock-in rats via microinjection of TALE nucleases into fertilized eggs. This technology is an efficient, cost- and time-effective method for creating new rat models.
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inserm-02150568 , version 1 (07-06-2019)



Laurent Tesson, Severine Remy, Séverine Ménoret, Claire Usal, Reynald Thinard, et al.. Genome Editing in Rats Using TALE Nucleases. TALENs. Methods in Molecular Biology, pp.245-259, 2016, ⟨10.1007/978-1-4939-2932-0_18⟩. ⟨inserm-02150568⟩
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