, Transpl Int, pp.24-26, 2011.
, Sci Transl Med, issue.2, pp.17-27, 2010.
, Am J Transplant, vol.15, pp.88-100, 2015.
, J Immunol, vol.2015, pp.1454-1466
, PLoS ONE, issue.8, p.83139, 2013.
, Exp Dermatol, vol.17, pp.703-712, 2008.
, Br J Dermatol, vol.164, pp.670-672, 2011.
, J Immunol, vol.182, pp.5836-5845, 2009.
, Br J Dermatol, vol.172, pp.345-353, 2014.
, Representative skin erythema from a control excipient-treated animal (left) and a FR104-treated baboon (right) after 5 days of topical Aldara application on skin. (b) Skin redness score (left), skin thickness (middle) and scaling core (right) measured over time of baboons treated with a single injection of excipient, Figure 1. Selective CD28 blockade prevents Aldara-induced skin inflammation. (a)
, A second and third challenge of 14 days of Aldara application were performed 2 months and 5 months after the single injection of the drug. (c) mRNA transcripts quantification by qPCR on skin biopsies performed on na? ?ve animals (n = 3) and excipient-treated animals (n = 3) at days 5, 12 and 18 after beginning of Aldara application. HPRT was used as housekeeping gene. Data are mean AE SEM. Skin redness, thickness and scaling scores were measured by at least two observers, mg/kg of FR104 (blue, n = 3) 24 h before topical application of Aldara for 14 days
, CD3, CD68, CD8 and nucleus (blue) staining of na? ?ve skin (top) or skin biopsies performed 18 days after start of Aldara topical application on baboons treated with excipient (middle) or 10 mg/kg of FR104 (bottom). Ki-67 staining was performed on na? ?ve skin or skin biopsies harvested at day 12 after Aldara application, Figure 2. Selective CD28 blockade prevents skin inflammatory infiltrates. Representative Haematoxylin-eosin-safran (HES)