Human Neonatal Hepatocyte Transplantation Induces Long-Term Rescue of Unconjugated Hyperbilirubinemia in the Gunn Rat

Abstract : Crigler-Najjar type 1 disease is a rare inherited metabolic disease characterized by high levels of unconjugated bilirubin due to the complete absence of hepatic uridine diphosphoglucuronate-glucuronosyltransferase activity. Hepatocyte transplanta-tion (HT) has been proposed as an alternative treatment for Crigler-Najjar syndrome, but it is still limited by the quality and the low engraftment and repopulation ability of the cells used. Because of their attachment capability and expression of adhesion molecules as well as the higher proportion of hepatic progenitor cells, neonatal hepatocytes may have an advantage over adult cells. Adult or neonatal hepatocytes were transplanted into Gunn rats, a model for Crigler-Najjar disease. Engraftment and repopulation were studied and compared by immunofluorescence (IF). Additionally, the serum bilirubin levels , the presence of bilirubin conjugates in rat serum, and the expression of uridine diphosphate glucuronosyltransferase 1 family polypeptide A1 (UGT1A1) in rat liver samples were also analyzed. Here we show that neonatal HT results in long-term correction in Gunn rats. In comparison with adult cells, neonatal cells showed better engraftment and repopulation capability 3 days and 6 months after transplantation, respectively. Bilirubinemia decreased in the transplanted animals during the whole experimental follow-up (6 months). Bilirubin conjugates were also present in the serum of the transplanted Additional Supporting Information may be found in the online version of this article.
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Laia Tolosa, Silvia Lopez, Eugenia Pareja, Maria Donato, Anne Myara, et al.. Human Neonatal Hepatocyte Transplantation Induces Long-Term Rescue of Unconjugated Hyperbilirubinemia in the Gunn Rat. Liver Transplantation, Wiley, 2015, 21 (6), pp.801-811. ⟨10.1002/lt.24121⟩. ⟨inserm-02147047⟩

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