Cell competition during reprogramming gives rise to dominant clones

Abstract : The ability to generate induced pluripotent stem cells from differentiated cell types has enabled researchers to engineer cell states. Although studies have identified molecular networks that reprogram cells to pluripotency, the cellular dynamics of these processes remain poorly understood. Here, by combining cellular barcoding, mathematical modeling, and lineage tracing approaches, we demonstrate that reprogramming dynamics in heterogeneous populations are driven by dominant “elite” clones. Clones arise a priori from a population of poised mouse embryonic fibroblasts derived from Wnt1-expressing cells that may represent a neural crest–derived population.This work highlights the importance of cellular dynamics in fate programming outcomes and uncovers cell competition as a mechanism by which cells with eliteness emerge to occupy and dominate the reprogramming niche.
Document type :
Journal articles
Complete list of metadatas

Cited literature [14 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-02145973
Contributor : Ana Paula Dutra Azevedo <>
Submitted on : Monday, June 3, 2019 - 2:58:52 PM
Last modification on : Friday, November 15, 2019 - 8:44:07 AM

File

 Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed until : jamais

Please log in to resquest access to the document

Identifiers

Collections

Citation

Nika Shakiba, Ahmed Fahmy, Gowtham Jayakumaran, Sophie Mcgibbon, Laurent David, et al.. Cell competition during reprogramming gives rise to dominant clones. Science, American Association for the Advancement of Science, 2019, 364 (6438), Epub 2019 Mar 21. ⟨10.1126/science.aan0925⟩. ⟨inserm-02145973⟩

Share

Metrics

Record views

36