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Journal articles
Cell competition during reprogramming gives rise to dominant clones
Nika Shakiba
1
Ahmed Fahmy
2
Gowtham Jayakumaran
2, 3
Sophie Mcgibbon
4
Laurent David
5, 6, 7
Daniel Trcka
3
Judith Elbaz
3
Mira Puri
3, 8
Andras Nagy
3, 9, 10, 11
Derek van der Kooy
2, 12
Sidhartha Goyal
1, 4
Jeffrey Wrana
2, 3
Peter Zandstra
1, 12, 13, 14, 15, *
*
Corresponding author
5
P2R - INSERM UMS016/CNRS UMS3556/UN FED4203 - Plateforme "Production de protéines recombinantes"
SFR François Bonamy - Structure fédérative de recherche François Bonamy
7
Team 2 - U1064 Inserm - CRTI - Genetic and Cellular Engineering in Immunology and Regenerative Medicine
U1064 Inserm - CRTI - Centre de Recherche en Transplantation et Immunologie
Laurent David 5, 6, 7
Author IdHAL : laurentdavid ORCID : https://orcid.org/0000-0003-3632-8537
Daniel Trcka 3
Author
Judith Elbaz 3
Author
Mira C Puri 3, 8
Author
Andras Nagy 3, 9, 10, 11
Author
Derek van der Kooy 2, 12
Author
Sidhartha Goyal 1, 4
Author
Jeffrey L Wrana 2, 3
Author
Peter W Zandstra 1, 12, 13, 14, 15
Correspondent author
1
IBBME - Institute of Biomaterials and Biomedical Engineering [Ontario, Canada] (5 King's College Road, Toronto, Ontario - Canada)
- University of Toronto (27 King's College Circle, Toronto M5S 1A1 - Canada)
2
Department of Molecular Genetics [Toronto] (1 King's College Circle, Toronto, Ontario M5S 1A8 - Canada)
- University of Toronto (27 King's College Circle, Toronto M5S 1A1 - Canada)
3
Lunenfeld-Tanenbaum Research Institute [Toronto, Canada] (Sinai Health System, 600 University Ave., Toronto, ON, Canada - Canada)
4
Department of Physics [Toronto] ( 60 St. George St., Toronto, Ontario, M5S 1A7 - Canada)
- University of Toronto (27 King's College Circle, Toronto M5S 1A1 - Canada)
5
P2R - INSERM UMS016/CNRS UMS3556/UN FED4203 - Plateforme "Production de protéines recombinantes" (France)
- SFR François Bonamy - Structure fédérative de recherche François Bonamy (8, quai Moncousu - BP 70721 - 44007 Nantes Cedex 1 - )
- UN - Université de Nantes (1, quai de Tourville - BP 13522 - 44035 Nantes cedex 1 - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : UMS 016 (101, rue de Tolbiac, 75013 Paris - France)
- CNRS - Centre National de la Recherche Scientifique : UMS 3556 (France)
- IRS-UN - Institut de Recherche en Santé de l'Université de Nantes : FED 4203 (8 Quai Moncousu 44007 Nantes - France)
6
ITUN - Institut de transplantation urologie-néphrologie (France)
- UN - Université de Nantes (1, quai de Tourville - BP 13522 - 44035 Nantes cedex 1 - France)
- CHU Nantes - Centre hospitalier universitaire de Nantes (1 Place Alexis-Ricordeau, 44000 Nantes - France)
7
Team 2 - U1064 Inserm - CRTI - Genetic and Cellular Engineering in Immunology and Regenerative Medicine (CHU Nantes - Hôtel Dieu - 30 Bd Jean Monnet 44093 Nantes Cedex 01. - France)
- U1064 Inserm - CRTI - Centre de Recherche en Transplantation et Immunologie (CHU Nantes - Hôtel Dieu - 30 Bd Jean Monnet 44093 Nantes Cedex 01. - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U1064 (101, rue de Tolbiac, 75013 Paris - France)
- UFR MEDECINE - Université de Nantes - UFR de Médecine et des Techniques Médicales (1 rue Gaston Veil BP 53508 44035 NANTES Cedex 1 - France)
- UN - Université de Nantes (1, quai de Tourville - BP 13522 - 44035 Nantes cedex 1 - France)
8
MBP - Department of Medical Biophysics (Department of Medical Biophysics University of Toronto Ontario Cancer Institute, Princess Margaret Hospital 610 University Avenue, Rm 7-411 Toronto, Ontario, CANADA - Canada)
- University of Toronto (27 King's College Circle, Toronto M5S 1A1 - Canada)
9
Department of Obstetrics and Gynecology [Toronto, ON, Canada] (Canada)
- University of Toronto (27 King's College Circle, Toronto M5S 1A1 - Canada)
10
Institute of Medical Science [Toronto] (Faculty of Medicine, University of Toronto
Medical Sciences Building
1 King’s College Circle, Room 2374
Toronto, ON M5S 1A8 - Canada)
- University of Toronto (27 King's College Circle, Toronto M5S 1A1 - Canada)
11
Australian Regenerative Medicine Institute [Melbourne, Australia] (Australia)
- Monash University [Melbourne] (Victoria 3800, Australia - Australia)
12
CCBR - The Donnelly Centre for Cellular and Biomolecular Research [Toronto, ON, Canada] (Canada)
- University of Toronto (27 King's College Circle, Toronto M5S 1A1 - Canada)
13
School of Biomedical Engineering [Vancouver, Canada] (Canada)
- UBC - University of British Columbia (Vancouver Campus, , 2329 West Mall, Vancouver, BC, V6T 1Z4 / Okanagan Campus, 3333 University Way, Kelowna, BC, V1V 1V7 - Canada)
14
The Biomedical Research Centre [Vancouver, Canada] (Canada)
- UNBC - University of Northern British Columbia [Prince George] (Prince George, BC, Canada V2N 4Z9 - Canada)
15
Michael Smith Laboratories [Vancouver, Canada] (Canada)
- UBC - University of British Columbia (Vancouver Campus, , 2329 West Mall, Vancouver, BC, V6T 1Z4 / Okanagan Campus, 3333 University Way, Kelowna, BC, V1V 1V7 - Canada)
Abstract : The ability to generate induced pluripotent stem cells from differentiated cell types has enabled researchers to engineer cell states. Although studies have identified molecular networks that reprogram cells to pluripotency, the cellular dynamics of these processes remain poorly understood. Here, by combining cellular barcoding, mathematical modeling, and lineage tracing approaches, we demonstrate that reprogramming dynamics in heterogeneous populations are driven by dominant “elite” clones. Clones arise a priori from a population of poised mouse embryonic fibroblasts derived from Wnt1-expressing cells that may represent a neural crest–derived population.This work highlights the importance of cellular dynamics in fate programming outcomes and uncovers cell competition as a mechanism by which cells with eliteness emerge to occupy and dominate the reprogramming niche.
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Journal articles
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https://www.hal.inserm.fr/inserm-02145973
Contributor : Sylvie Le Bihan <>
Submitted on : Monday, June 3, 2019 - 2:58:52 PM
Last modification on : Friday, December 18, 2020 - 5:30:04 PM
Contributor : Sylvie Le Bihan <>
Submitted on : Monday, June 3, 2019 - 2:58:52 PM
Last modification on : Friday, December 18, 2020 - 5:30:04 PM
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Identifiers
- HAL Id : inserm-02145973, version 1
- PUBMED : 30898844
- DOI : 10.1126/science.aan0925
Collections
INSERM | CNRS | UNIV-NANTES | CRTI
Citation
Nika Shakiba, Ahmed Fahmy, Gowtham Jayakumaran, Sophie Mcgibbon, Laurent David, et al.. Cell competition during reprogramming gives rise to dominant clones. Science, American Association for the Advancement of Science, 2019, 364 (6438), Epub 2019 Mar 21. ⟨10.1126/science.aan0925⟩. ⟨inserm-02145973⟩
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