, AB19808 (anti-collagen IV) was purchased from abcam. Sc19982 (anti-VCAM-1) was purchased from, anti-CD31/ PECAM), 4E3 (anti-CD21/35), ALY7 (anti-Lyve-1), MDJ7/18 (anti-CD105), and IK22/5 (anti CD254/RANK-L) were purchased from e-bioscience, vol.390, pp.11-26

, These antibodies were visualized by direct coupling to Pacific blue, allophycocyanin, Alexa fluor-488, -568, and -647, or through the use of alexa-fluor TSA kits or Alexa fluor -488, and RTD4E10 (anti-GP38/Podoplanin) was purchased from ReliaTech, vol.568, pp.17-26

, DT Treatment Diphteria toxin was purchased from Calbiochem (Merck KGaA

C. D. Allen and J. G. Cyster, Follicular dendritic cell networks of primary follicles and germinal centers: phenotype and function, Semin Immunol, vol.20, pp.14-25, 2008.

J. E. Gretz, A. O. Anderson, and S. Shaw, Cords, channels, corridors and conduits: critical architectural elements facilitating cell interactions in the lymph node cortex, Immunol Rev, vol.156, pp.11-24, 1997.
DOI : 10.1111/j.1600-065x.1997.tb00955.x

S. N. Mueller and R. N. Germain, Stromal cell contributions to the homeostasis and functionality of the immune system, Nat Rev Immunol, vol.9, pp.618-629, 2009.

M. Bajenoff, J. G. Egen, L. Y. Koo, J. P. Laugier, and F. Brau, Stromal cell networks regulate lymphocyte entry, migration, and territoriality in lymph nodes, Immunity, vol.25, pp.989-1001, 2006.
URL : https://hal.archives-ouvertes.fr/hal-00316442

R. Munoz-fernandez, F. J. Blanco, C. Frecha, F. Martin, and M. Kimatrai, Follicular dendritic cells are related to bone marrow stromal cell progenitors and to myofibroblasts, J Immunol, vol.177, pp.280-289, 2006.
DOI : 10.4049/jimmunol.177.1.280

URL : http://www.jimmunol.org/content/177/1/280.full.pdf

P. Schneider and J. Tschopp, BAFF and the regulation of B cell survival, Immunol Lett, vol.88, pp.57-62, 2003.

A. Link, T. K. Vogt, S. Favre, M. R. Britschgi, and H. Acha-orbea, Fibroblastic reticular cells in lymph nodes regulate the homeostasis of naive T cells, Nat Immunol, vol.8, pp.1255-1265, 2007.

K. M. Ansel, V. N. Ngo, P. L. Hyman, S. A. Luther, and R. Forster, A chemokine-driven positive feedback loop organizes lymphoid follicles, Nature, vol.406, pp.309-314, 2000.
DOI : 10.1038/35018581

R. Forster, A. Schubel, D. Breitfeld, E. Kremmer, and I. Renner-muller, CCR7 coordinates the primary immune response by establishing functional microenvironments in secondary lymphoid organs, Cell, vol.99, pp.23-33, 1999.

X. Wang, B. Cho, K. Suzuki, Y. Xu, and J. A. Green, Follicular dendritic cells help establish follicle identity and promote B cell retention in germinal centers, J Exp Med, vol.208, issue.12, pp.2497-2510, 2011.
DOI : 10.1084/jem.20111449

URL : http://jem.rupress.org/content/208/12/2497.full.pdf

M. Miyasaka and T. Tanaka, Lymphocyte trafficking across high endothelial venules: dogmas and enigmas, Nat Rev Immunol, vol.4, pp.360-370, 2004.
DOI : 10.1038/nri1354

W. Cl, Normal structure, function, and histology of lymph nodes, Toxicol Pathol, vol.34, pp.409-424, 2006.

A. J. Young, The physiology of lymphocyte migration through the single lymph node in vivo, Semin Immunol, vol.11, pp.73-83, 1999.

M. Kraus, M. B. Alimzhanov, N. Rajewsky, and K. Rajewsky, Survival of resting mature B lymphocytes depends on BCR signaling via the Igalpha/beta heterodimer, Cell, vol.117, pp.787-800, 2004.

H. Luche, O. Weber, N. Rao, T. Blum, C. Fehling et al., Faithful activation of an extra-bright red fluorescent protein in ''knock-in'' Cre-reporter mice ideally suited for lineage tracing studies, Eur J Immunol, vol.37, pp.43-53, 2007.

K. Takahashi, Y. Kozono, T. J. Waldschmidt, D. Berthiaume, and R. J. Quigg, Mouse complement receptors type 1 (CR1;CD35) and type 2 (CR2;CD21): expression on normal B cell subpopulations and decreased levels during the development of autoimmunity in MRL/lpr mice, J Immunol, vol.159, pp.1557-1569, 1997.

P. Victoratos, J. Lagnel, S. Tzima, M. B. Alimzhanov, and K. Rajewsky, FDC-specific functions of p55TNFR and IKK2 in the development of FDC networks and of antibody responses, Immunity, vol.24, pp.65-77, 2006.

T. Katakai, T. Hara, J. H. Lee, H. Gonda, and M. Sugai, A novel reticular stromal structure in lymph node cortex: an immuno-platform for interactions among dendritic cells, T cells and B cells, Int Immunol, vol.16, pp.1133-1142, 2004.

A. Cerny, R. M. Zinkernagel, and P. Groscurth, Development of follicular dendritic cells in lymph nodes of B-cell-depleted mice, Cell Tissue Res, vol.254, pp.449-454, 1988.

Y. X. Fu, G. Huang, Y. Wang, and D. D. Chaplin, B lymphocytes induce the formation of follicular dendritic cell clusters in a lymphotoxin alpha-dependent fashion, J Exp Med, vol.187, pp.1009-1018, 1998.

Z. F. Kapasi, D. Qin, W. G. Kerr, M. H. Kosco-vilbois, and L. D. Shultz, Follicular dendritic cell (FDC) precursors in primary lymphoid tissues, J Immunol, vol.160, pp.1078-1084, 1998.

M. Bajenoff and R. N. Germain, B-cell follicle development remodels the conduit system and allows soluble antigen delivery to follicular dendritic cells, Blood, vol.114, pp.4989-4997, 2009.
URL : https://hal.archives-ouvertes.fr/hal-00432044

N. J. Krautler, V. Kana, J. Kranich, Y. Tian, and D. Perera, Follicular dendritic cells emerge from ubiquitous perivascular precursors, Cell, vol.150, pp.194-206, 2012.
DOI : 10.1016/j.cell.2012.05.032

URL : https://doi.org/10.1016/j.cell.2012.05.032

T. Katakai, Marginal reticular cells: a stromal subset directly descended from the lymphoid tissue organizer, Front Immunol, vol.3, 0200.
DOI : 10.3389/fimmu.2012.00200

URL : https://www.frontiersin.org/articles/10.3389/fimmu.2012.00200/pdf

I. C. Maclennan, Germinal centers, Annu Rev Immunol, vol.12, pp.117-139, 1994.

C. D. Allen, T. Okada, and J. G. Cyster, Germinal-center organization and cellular dynamics, Immunity, vol.27, pp.190-202, 2007.
DOI : 10.1016/j.immuni.2007.07.009

URL : https://doi.org/10.1016/j.immuni.2007.07.009

R. Roozendaal, T. R. Mempel, L. A. Pitcher, S. F. Gonzalez, and A. Verschoor, Conduits mediate transport of low-molecular-weight antigen to lymph node follicles, Immunity, vol.30, pp.264-276, 2009.

R. Endres, M. B. Alimzhanov, T. Plitz, A. Futterer, and M. H. Kosco-vilbois, Mature follicular dendritic cell networks depend on expression of lymphotoxin beta receptor by radioresistant stromal cells and of lymphotoxin beta and tumor necrosis factor by B cells, J Exp Med, vol.189, pp.159-168, 1999.

T. Buch, F. L. Heppner, C. Tertilt, T. J. Heinen, and M. Kremer, A Creinducible diphtheria toxin receptor mediates cell lineage ablation after toxin administration, Nat Methods, vol.2, pp.419-426, 2005.
DOI : 10.1038/nmeth762

M. Gonzalez, F. Mackay, J. L. Browning, M. H. Kosco-vilbois, and R. J. Noelle, The sequential role of lymphotoxin and B cells in the development of splenic follicles, J Exp Med, vol.187, pp.997-1007, 1998.

A. Tumanov, D. Kuprash, M. Lagarkova, S. Grivennikov, and K. Abe, Distinct role of surface lymphotoxin expressed by B cells in the organization of secondary lymphoid tissues, Immunity, vol.17, pp.239-250, 2002.

V. N. Ngo, H. Korner, M. D. Gunn, K. N. Schmidt, and D. S. Riminton, Lymphotoxin alpha/beta and tumor necrosis factor are required for stromal cell expression of homing chemokines in B and T cell areas of the spleen, J Exp Med, vol.189, pp.403-412, 1999.

T. Okada, V. N. Ngo, E. H. Ekland, R. Forster, and M. Lipp, Chemokine requirements for B cell entry to lymph nodes and Peyer's patches, J Exp Med, vol.196, pp.65-75, 2002.
DOI : 10.1084/jem.20020201

URL : http://jem.rupress.org/content/jem/196/1/65.full.pdf

C. Park, I. Y. Hwang, R. K. Sinha, O. Kamenyeva, and M. D. Davis, Lymph node B lymphocyte trafficking is constrained by anatomy and highly dependent upon chemoattractant desensitization, Blood, vol.119, pp.978-989, 2012.
DOI : 10.1182/blood-2011-06-364273

URL : http://www.bloodjournal.org/content/119/4/978.full.pdf

P. A. Koni, R. Sacca, P. Lawton, J. L. Browning, and N. H. Ruddle, Distinct roles in lymphoid organogenesis for lymphotoxins alpha and beta revealed in lymphotoxin beta-deficient mice, Immunity, vol.6, pp.491-500, 1997.
DOI : 10.1016/s1074-7613(00)80292-7

URL : https://doi.org/10.1016/s1074-7613(00)80292-7

M. Bajenoff, S. Granjeaud, and S. Guerder, The strategy of T cell antigenpresenting cell encounter in antigen-draining lymph nodes revealed by imaging of initial T cell activation, J Exp Med, vol.198, pp.715-724, 2003.