Ex vivo evolution of human antibodies by CRISPR-X: from a naive B cell repertoire to affinity matured antibodies

Marie-Claire Devilder; Melinda Moyon; Laetitia Gautreau-Rolland; Benjamin Navet; Jeanne Perroteau; Florent Delbos; Marie-Claude Gesnel; Richard Breathnach; Xavier Saulquin

doi:10.1186/s12896-019-0504-z

Journal articles

Ex vivo evolution of human antibodies by CRISPR-X: from a naive B cell repertoire to affinity matured antibodies

Marie-Claire Devilder ^{1, 2, 3} Melinda Moyon ^{1, 2} Laetitia Gautreau-Rolland ^{1, 2} Benjamin Navet ^{1, 2} Jeanne Perroteau ^{1, 2} Florent Delbos ⁴ Marie-Claude Gesnel ^{1, 2, 3} Richard Breathnach ^{1, 2, *} Xavier Saulquin ^{1, 2, *}

* Corresponding author

1 CRCINA-ÉQUIPE 1 - Immunobiology of Human αβ and γδ T Cells and Immunotherapeutic Applications

CRCINA - Centre de Recherche en Cancérologie et Immunologie Nantes-Angers

2 LabEX IGO Immunothérapie Grand Ouest

3 CHU Hôtel-Dieu - Centre Hospitalier Universiatire Hôtel-Dieu de Nantes

4 Etablissement Français du Sang [Nantes]

Abstract : Methodology article

Keywords : Human antibodies SHM CRISPR CRISPR-X AID Cytofluorimetry HLA Tetramers

Document type :

Journal articles

Domain :

Life Sciences [q-bio] / Cancer

Life Sciences [q-bio] / Genetics

Complete list of metadata

Cited literature [36 references] Display Hide Download

https://www.hal.inserm.fr/inserm-02117156
Contributor : Elizabeth Bernardo Connect in order to contact the contributor
Submitted on : Thursday, May 2, 2019 - 9:20:27 AM
Last modification on : Wednesday, April 27, 2022 - 3:54:05 AM

Ex vivo evolution of human antibodies by CRISPR-X: from a naive B cell repertoire to affinity matured antibodies

File

Identifiers

Collections

Citation

Export

Metrics

91

78

Ex vivo evolution of human antibodies by CRISPR-X: from a naive B cell repertoire to affinity matured antibodies

File

Identifiers

Collections

Citation

Export

Share

Metrics

91

78