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Journal articles
Assessment of seven protocols to prepare Cutibacterium acnes bacterial lysates to measure its immunogenic potential and review of the literature
Abstract : Seven protocols were tested to prepare cell wall extracts from live Cutibacterium acnes. Different parameters were modified: thawing/freezing and sonication/freezing cycles, to impact on mechanical degradation of the bacteria. Finally, the immunogenic potential of the extracts generated was evaluated by measuring IL-8 releases using an in vitro skin explants system. The aim of this article was to compare the existing protocols from the scientific literature, and also propose a standardized method developed in our facilities.
Cited literature [23 references]
https://www.hal.inserm.fr/inserm-02117150
Contributor : Elizabeth Bernardo Connect in order to contact the contributor
Submitted on : Thursday, May 2, 2019 - 9:08:23 AM
Last modification on : Wednesday, November 3, 2021 - 4:36:54 AM
Contributor : Elizabeth Bernardo Connect in order to contact the contributor
Submitted on : Thursday, May 2, 2019 - 9:08:23 AM
Last modification on : Wednesday, November 3, 2021 - 4:36:54 AM