Use of MALDI-TOF MS for the Identification of Chad Mosquitoes and the Origin of Their Blood Meal - Archive ouverte HAL Access content directly
Journal Articles American Journal of Tropical Medicine and Hygiene Year : 2019

Use of MALDI-TOF MS for the Identification of Chad Mosquitoes and the Origin of Their Blood Meal

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Abstract

Matrix-assisted desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a clinical microbiology tool for the systematic identification of microorganisms. It has recently been presented as an innovative tool for the rapid and accurate identification of mosquitoes and their blood meal. To evaluate the capacity of this tool to identify mosquitoes collected in a tropical environment and preserved with silica gel, we analyzed 188 mosquitoes of different species collected in Chad, which were preserved with silica gel for 2 months. The MALDI-TOF MS analysis correctly identified 96% of the mosquitoes and 37.5% of their blood meals. Using MALDI-TOF MS and molecular biology, eight mosquito species were identified, including Anopheles gambiae s.l., Anopheles rufipes, Culex quinquefasciatus, Culex neavei, Culex pipiens, Culex perexiguus, Culex rima, and Culex watti. Blood meal identification revealed that mosquitoes fed mainly on humans, birds, and cows. Matrix-assisted desorption/ionization time-of-flight mass spectrometry appears to be a promising, fast, and reliable tool to identify mosquitoes and the origin of their blood meal for samples stored with silica gel.

Dates and versions

inserm-02090182 , version 1 (04-04-2019)

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Adama Zan Diarra, Maureen Laroche, Franck Berger, Philippe Parola. Use of MALDI-TOF MS for the Identification of Chad Mosquitoes and the Origin of Their Blood Meal. American Journal of Tropical Medicine and Hygiene, 2019, 100 (1), pp.47-53. ⟨10.4269/ajtmh.18-0657⟩. ⟨inserm-02090182⟩
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