Dry-heat inactivation of “Mycobacterium canettii”

Abstract : OBJECTIVE: "Mycobacterium canettii" is responsible for non-transmissible lymph node and pulmonary tuberculosis in persons exposed in the Horn of Africa. In the absence of direct human transmission, contaminated water and foodstuffs could be sources of contamination. We investigated the dry-heat inactivation of "M. canettii" alone and mixed into mock-infected foodstuffs by inoculating agar cylinders and milk with 104 colony-forming units of "M. canettii" CIPT140010059 and two "M. canettii" clinical strains with Mycobacterium tuberculosis H37Rv as a control. RESULTS: Exposed to 35 °C, M. tuberculosis H37Rv, "M canettii" CIPT140010059 and "M. canettii" 157 exhibited a survival rate of 108, 95 and 81%, which is significantly higher than that of "M. canettii" 173. However, all tested mycobacteria tolerated a 90-min exposure at 45 °C. In the foodstuff models set at 70 °C, no growing mycobacteria were visualized. This study supports the premise that "M. canettii" may survive up to 45 °C; and suggests that contaminated raw drinks and foodstuffs but not cooked ones may be sources of infection for populations.
Document type :
Journal articles
Complete list of metadatas

Cited literature [23 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-02076394
Contributor : Myriam Bodescot <>
Submitted on : Friday, March 22, 2019 - 10:07:07 AM
Last modification on : Saturday, March 23, 2019 - 1:27:33 AM
Long-term archiving on : Sunday, June 23, 2019 - 1:23:23 PM

File

13104_2017_Article_2522.pdf
Publisher files allowed on an open archive

Identifiers

Collections

Citation

Djaltou Osman, Eric Garnotel, Michel Drancourt. Dry-heat inactivation of “Mycobacterium canettii”. BMC Research Notes, BioMed Central, 2017, 10 (1), pp.201. ⟨10.1186/s13104-017-2522-z⟩. ⟨inserm-02076394⟩

Share

Metrics

Record views

90

Files downloads

67