Efficient delivery of Cre-recombinase to neurons in vivo and stable transduction of neurons using adeno-associated and lentiviral vectors, BMC Neurosci, vol.5, p.4, 2004. ,
Effects of bilateral injection of GABA into the substantia nigra on spontaneous behavior and measures of analgesia, Neuropharmacology, vol.27, pp.817-821, 1988. ,
,
Prolonged Cre expression driven by the ?-myosin heavy chain promoter can be cardiotoxic, J. Mol. Cell. Cardiol, vol.86, pp.54-61, 2015. ,
A non-specific effect associated with conditional transgene expression based on Cre-loxP strategy in mice, PloS One, vol.6, 2011. ,
Focal deletion of the adenosine A1 receptor in adult mice using an adeno-associated viral vector, J. Neurosci, vol.23, pp.5762-5770, 2003. ,
Illegitimate Credependent chromosome rearrangements in transgenic mouse spermatids, Proc. Natl. Acad. Sci. U S A, vol.97, pp.13702-13707, 2000. ,
Selective autophagy as a potential therapeutic target for neurodegenerative disorders, Lancet Neurol, vol.17, pp.802-815, 2018. ,
p53: traffic cop at the crossroads of DNA repair and recombination, Nature Rev. Mol. Cell Biol, vol.6, pp.44-55, 2005. ,
Mesolimbic leptin signaling negatively regulates cocaine-conditioned reward, Transl. Psychiatry, vol.6, p.972, 2016. ,
Self-excising retroviral vectors encoding the Cre recombinase overcome Cre-mediated cellular toxicity, Mol. Cell, vol.8, pp.233-243, 2001. ,
Thigmotaxis as an index of anxiety in mice. Influence of dopaminergic transmissions, Behav. Brain Res, vol.61, pp.59-64, 1994. ,
The apoptotic ring: a novel entity with phosphorylated histones H2AX and H2B and activated DNA damage response kinases, Cell Cycle, vol.8, pp.1853-1859, 2009. ,
Lewy body-like alpha-synuclein aggregates resist degradation and impair macroautophagy, J. Biol. Chem, vol.288, pp.15194-15210, 2013. ,
Apoptosis: controlled demolition at the cellular level, Nat. Rev. Mol. Cell Biol, vol.9, pp.231-241, 2008. ,
Evidence for baseline retinal pigment Accepted Article This article is protected by copyright, 2012. ,
, MannWhitney test, unpaired, ** p<0.01, *** p<0.001. (B) AAV-mediated Cre expression in the SN of PMAT fl/fl mice (n=24-25 per group) and WT mice (n=6 per group) did not modify anxiety level in the light dark box). (C) AAV-mediated Cre expression in the SN of PMAT fl/fl and WT mice disrupted locomotor sensitization to cocaine (10 mg/kg). Two-way analysis of variance (ANOVA), High thigmotaxis and impaired response to cocaine induced by AAV-mediated Cre expression in the SN of mice. (A)
, 0007) for PMAT fl/fl mice (n=7-8 per group
, 0001) and day of treatment/AAV type interaction
, Over time, Sidak's post-hoc test showed stronger locomotor sensitization for AAV-eGFP-injected mice than for AAV-eGFP-Cre-injected mice (* p<0.05, ** p<0.01*** p<0.001, compared to day 1). Values are given as mean ± s.e.m
, Mann-Whitney test, unpaired, *** p<0.0001. Representative images are shown on the left. Scale bars represent 100 µm. (B) AAV-mediated Cre expression in the SN of PMAT fl/fl mice (n=9 per group) and WT mice (n=6 per group) reduced dopamine transporter (DAT) immunofluorescence in dorsal striata of PMAT fl/fl mice (n=9 SN injections per group) and WT mice (n=6 SN injections per group). Intensity is expressed as percentage of mean values for AAV-eGFP controls. DLS, dorsolateral striatum; DMS, dorsomedial striatum
, AAV-mediated Cre expression in WT mice (n=6 mice per group) increased the number of cells positive for S439 phosphorylated H2AX (A; n=12 SN injections per group) and for K283 acetylated p53 (B; n=8-9 SN injections per group) in the SN of WT mice. Inset in A shows higher magnification of S439 phosphorylated H2AX apoptotic rings in AAV-eGFP-Cre injected mice. Scale bars represent 50 µm. Student's t-test, unpaired, *** p<0.001. (C) AAV-mediated Cre expression did not alter labeling for D175-cleaved caspase 3 (Cas3), in the SN of WT mice (top; (n=6 mice per group), DNA break and programmed cell death markers induced by AAV