A. Loyter, G. A. Scangos, and F. H. Ruddle, Mechanisms of DNA uptake by mammalian cells: fate of exogenously added DNA monitored by the use of fluorescent dyes, Proc. Natl. Acad. Sci. U.S.A, vol.79, pp.422-426, 1982.

J. Zabner, A. J. Fasbender, T. Moninger, K. A. Poellinger, and M. J. Welsh, Cellular and molecular barriers to gene transfer by a cationic lipid, J. Biol. Chem, vol.270, pp.18997-19007, 1995.

G. Orphanides and D. Reinberg, RNA polymerase II elongation through chromatin, Nature, vol.407, pp.471-475, 2000.

X. Darzacq, Y. Shav-tal, V. De-turris, Y. Brody, S. M. Shenoy et al., In vivo dynamics of RNA polymerase II transcription, Nat .Struct. Mol. Biol, vol.14, pp.796-806, 2007.

D. Raab, M. Graf, F. Notka, T. Schodl, and R. Wagner, The GeneOptimizer Algorithm: using a sliding window approach to cope with the vast sequence space in multiparameter DNA sequence optimization, Syst. Synth. Biol, vol.4, pp.215-225, 2010.

A. Das, Control of transcription termination by RNA-binding proteins, Annu. Rev. Biochem, vol.62, pp.893-930, 1993.

R. J. Austin, T. Xia, J. Ren, T. T. Takahashi, and R. W. Roberts, Differential modes of recognition in N peptide-boxB complexes, Biochemistry, vol.42, pp.14957-14967, 2003.

F. Peyrane, B. Selisko, E. Decroly, J. J. Vasseur, D. Benarroch et al., High-yield production of short GpppA-and 7MeGpppA-capped RNAs and HPLC-monitoring of methyltransfer reactions at the guanine-N7 and adenosine-2'O positions, Nucleic Acids Res, vol.35, p.26, 2007.
URL : https://hal.archives-ouvertes.fr/hal-00139133

M. Bouvet, I. Imbert, L. Subissi, L. Gluais, B. Canard et al., RNA 3'-end mismatch excision by the severe acute respiratory syndrome coronavirus nonstructural protein nsp10/nsp14 exoribonuclease complex, Proc. Natl. Acad. Sci. U.S.A, vol.109, pp.9372-9377, 2012.

A. L. Niles, R. A. Moravec, and T. L. Riss, In vitro viability and cytotoxicity testing and same-well multi-parametric combinations for high throughput screening, Curr. Chem. Genomics, vol.3, pp.33-41, 2009.

M. H. Cho, A. Niles, R. Huang, J. Inglese, C. P. Austin et al., A bioluminescent cytotoxicity assay for assessment of membrane integrity using a proteolytic biomarker, Toxicol. In Vitro, vol.22, pp.1099-1106, 2008.

A. Einhauer and A. Jungbauer, The FLAG peptide, a versatile fusion tag for the purification of recombinant proteins, J. Biochem. Biophys. Methods, vol.49, pp.455-465, 2001.

R. G. Chubet and B. L. Brizzard, Vectors for expression and secretion of FLAG epitope-tagged proteins in mammalian cells, Biotechniques, vol.20, pp.136-141, 1996.

S. Q. Jing, T. Spencer, K. Miller, C. Hopkins, and I. S. Trowbridge, Role of the human transferrin receptor cytoplasmic domain in endocytosis: localization of a specific signal sequence for internalization, J. Cell Biol, vol.110, pp.283-294, 1990.

S. D. Power, M. A. Lochrie, and R. O. Poyton, The nuclear-coded subunits of yeast cytochrome c oxidase. III. Identification of homologous subunits in yeast, bovine heart, and Neurospora crassa cytochrome c oxidases, J. Biol. Chem, vol.259, pp.6575-6578, 1984.

S. T. Smale and R. Tjian, T-antigen-DNA polymerase alpha complex implicated in simian virus 40 DNA replication, Mol. Cell Biol, vol.6, pp.4077-4087, 1986.

, Nucleic Acids Research, vol.47, issue.5, p.2697, 2019.

D. Wild, The Immunoassay Handbook: Theory and Applications of Ligand Binding, ELISA and Related Techniques, 2013.

N. Komatsu, H. Nakauchi, A. Miwa, T. Ishihara, M. Eguchi et al., Establishment and characterization of a human leukemic cell line with megakaryocytic features: dependency on granulocyte-macrophage colony-stimulating factor, interleukin 3, or erythropoietin for growth and survival, Cancer Res, vol.51, pp.341-348, 1991.

O. Hermine, A. Dubart, F. Porteux, P. Mayeux, M. Titeux et al., Inhibition of the erythropoietin-induced erythroid differentiation by granulocyte-macrophage colony-stimulating factor in the human UT-7 cell line is not due to a negative regulation of the erythropoietin receptor, Blood, vol.87, pp.1746-1753, 1996.

V. Skibeli, G. Nissen-lie, and P. Torjesen, Sugar profiling proves that human serum erythropoietin differs from recombinant human erythropoietin, Blood, vol.98, pp.3626-3634, 2001.

S. B. Verrier and O. Jean-jean, Complementarity between the mRNA 5' untranslated region and 18S ribosomal RNA can inhibit translation, RNA, vol.6, pp.584-597, 2000.

Y. W. Yin and T. A. Steitz, Structural basis for the transition from initiation to elongation transcription in T7 RNA polymerase, Science, vol.298, pp.1387-1395, 2002.

C. J. Wilusz, M. Wormington, and S. W. Peltz, The cap-to-tail guide to mRNA turnover, Nat. Rev. Mol. Cell Biol, vol.2, pp.237-246, 2001.

K. Dower and M. Rosbash, T7 RNA polymerase-directed transcripts are processed in yeast and link 3' end formation to mRNA nuclear export, RNA, vol.8, pp.686-697, 2002.

R. A. Ikeda, The efficiency of promoter clearance distinguishes T7 class II and class III promoters, J. Biol. Chem, vol.267, pp.11322-11328, 1992.

M. D. Been and G. S. Wickham, Self-cleaving ribozymes of hepatitis delta virus RNA, Eur. J. Biochem, vol.247, pp.741-753, 1997.

B. M. Benton, W. K. Eng, J. J. Dunn, F. W. Studier, R. Sternglanz et al., Signal-mediated import of bacteriophage T7 RNA polymerase into the Saccharomyces cerevisiae nucleus and specific transcription of target genes, Mol. Cell Biol, vol.10, pp.353-360, 1990.

T. R. Fuerst, E. G. Niles, F. W. Studier, and B. Moss, Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase, Proc. Natl. Acad. Sci. U.S.A, vol.83, pp.8122-8126, 1986.

K. A. Engleka, E. W. Lewis, and B. H. Howard, Mechanisms of replication-deficient vaccinia virus/T7 RNA polymerase hybrid expression: effect of T7 RNA polymerase levels and alpha-amanitin, 1998.

, Virology, vol.243, pp.331-339

T. R. Fuerst and B. Moss, Structure and stability of mRNA synthesized by vaccinia virus-encoded bacteriophage T7 RNA polymerase in mammalian cells. Importance of the 5' untranslated leader, J. Mol. Biol, vol.206, pp.333-348, 1989.

O. Elroy-stein and B. Moss, Cytoplasmic expression system based on constitutive synthesis of bacteriophage T7 RNA polymerase in mammalian cells, Proc. Natl. Acad. Sci. U.S.A, vol.87, pp.6743-6747, 1990.

P. X. Guo and B. Moss, Interaction and mutual stabilization of the two subunits of vaccinia virus mRNA capping enzyme coexpressed in Escherichia coli, Proc. Natl. Acad. Sci. U.S.A, vol.87, pp.4023-4027, 1990.

S. Shuman, M. Surks, H. Furneaux, and J. Hurwitz, Purification and characterization of a GTP-pyrophosphate exchange activity from vaccinia virions. Association of the GTP-pyrophosphate exchange activity with vaccinia mRNA guanylyltransferase. RNA (guanine-7-)methyltransferase complex (capping enzyme), J. Biol. Chem, vol.255, pp.11588-11598, 1980.

G. Monroy, E. Spencer, and J. Hurwitz, Characteristics of reactions catalyzed by purified guanylyltransferase from vaccinia virus, J. Biol. Chem, vol.253, pp.4490-4498, 1978.

R. F. Boone, M. J. Ensinger, and B. Moss, Synthesis of mRNA guanylyltransferase and mRNA methyltransferases in cells infected with vaccinia virus, J. Virol, vol.21, pp.475-483, 1977.

X. Mao and S. Shuman, Intrinsic RNA (guanine-7) methyltransferase activity of the vaccinia virus capping enzyme D1 subunit is stimulated by the D12 subunit. Identification of amino acid residues in the D1 protein required for subunit association and methyl group transfer, J. Biol. Chem, vol.269, pp.24472-24479, 1994.

B. J. Natalizio, N. D. Robson-dixon, and M. A. Garcia-blanco, The Carboxyl-terminal Domain of RNA Polymerase II Is Not Sufficient to Enhance the Efficiency of Pre-mRNA Capping or Splicing in the Context of a Different Polymerase, J Biol Chem, vol.284, pp.8692-8702, 2009.

S. Kaneko, C. Chu, A. J. Shatkin, and J. L. Manley, Human capping enzyme promotes formation of transcriptional R loops in vitro, Proc. Natl. Acad. Sci. U.S.A, vol.104, pp.17620-17625, 2007.

S. Mccracken, N. Fong, E. Rosonina, K. Yankulov, G. Brothers et al., ) 5'-Capping enzymes are targeted to pre-mRNA by binding to the phosphorylated carboxy-terminal domain of RNA polymerase II, Genes Dev, vol.11, pp.3306-3318, 1997.

A. Meinhart and P. Cramer, Recognition of RNA polymerase II carboxy-terminal domain by 3'-RNA-processing factors, Nature, vol.430, pp.223-226, 2004.

C. K. Ho and S. Shuman, Distinct roles for CTD Ser-2 and Ser-5 phosphorylation in the recruitment and allosteric activation of mammalian mRNA capping enzyme, Mol. Cell, vol.3, pp.405-411, 1999.

J. R. Moll, S. B. Ruvinov, I. Pastan, and C. Vinson, Designed heterodimerizing leucine zippers with a ranger of pIs and stabilities up to 10, M. Protein Sci, vol.10, issue.15, pp.649-655, 2001.

E. Decroly, F. Ferron, J. Lescar, and B. Canard, Conventional and unconventional mechanisms for capping viral mRNA, Nat. Rev. Microbiol, vol.10, pp.51-65, 2011.

J. Martinez-costas, G. Sutton, N. Ramadevi, and P. Roy, Guanylyltransferase and RNA 5'-triphosphatase activities of the purified expressed VP4 protein of bluetongue virus, J. Mol. Biol, vol.280, pp.859-866, 1998.

N. Ramadevi, N. J. Burroughs, P. P. Mertens, I. M. Jones, and P. Roy, Capping and methylation of mRNA by purified recombinant VP4 protein of bluetongue virus, Proc. Natl. Acad. Sci. U.S.A, vol.95, pp.13537-13542, 1998.

G. Sutton, J. M. Grimes, D. I. Stuart, and P. Roy, Bluetongue virus VP4 is an RNA-capping assembly line, Nat. Struct. Mol. Biol, vol.14, pp.449-451, 2007.

D. Benarroch, P. Smith, and S. Shuman, Characterization of a trifunctional mimivirus mRNA capping enzyme and crystal structure of the RNA triphosphatase domain, Structure, vol.16, pp.501-512, 2008.

L. Pena, R. J. Yanez, Y. Revilla, E. Vinuela, and M. L. Salas, African swine fever virus guanylyltransferase, Virology, vol.193, pp.319-328, 1993.

M. Larsen, N. Gunge, and F. Meinhardt, Kluyveromyces lactis killer plasmid pGKL2: evidence for a viral-like capping enzyme encoded by ORF3, Plasmid, vol.40, pp.243-246, 1998.

M. Tiggemann, S. Jeske, M. Larsen, and F. Meinhardt, Kluyveromyces lactis cytoplasmic plasmid pGKL2: heterologous expression of Orf3p and proof of guanylyltransferase and mRNA-triphosphatase activities, Yeast, vol.18, pp.815-825, 2001.

Y. I. Li, Y. J. Chen, Y. H. Hsu, and M. Meng, Characterization of the AdoMet-dependent guanylyltransferase activity that is associated with the N terminus of bamboo mosaic virus replicase, J. Virol, vol.75, pp.782-788, 2001.

Y. L. Huang, Y. H. Hsu, Y. T. Han, and M. Meng, mRNA guanylation catalyzed by the S-adenosylmethionine-dependent guanylyltransferase of bamboo mosaic virus, J. Biol. Chem, vol.280, pp.13153-13162, 2005.

T. M. Coleman, G. Wang, and F. Huang, Superior 5' homogeneity of RNA from ATP-initiated transcription under the T7 phi 2.5 promoter, Nucleic Acids Res, vol.32, p.14, 2004.

P. Cong and S. Shuman, Mutational analysis of mRNA capping enzyme identifies amino acids involved in GTP binding, enzyme-guanylate formation, and GMP transfer to RNA, Mol. Cell Biol, vol.15, pp.6222-6231, 1995.

R. Lavigne, W. D. Sun, and G. Volckaert, PHIRE, a deterministic approach to reveal regulatory elements in bacteriophage genomes, Bioinformatics, vol.20, pp.629-635, 2004.

C. Camacho, G. Coulouris, V. Avagyan, N. Ma, J. Papadopoulos et al., BLAST+: architecture and applications, BMC Bioinformatics, vol.10, p.421, 2009.

D. Scholl, S. Adhya, and C. R. Merril, Bacteriophage SP6 is closely related to phages K1-5, K5, and K1E but encodes a tail protein very similar to that of the distantly related P22, J. Bacteriol, vol.184, pp.2833-2836, 2002.

O. V. Makarova, E. M. Makarov, R. Sousa, and M. Dreyfus, Transcribing of Escherichia coli genes with mutant T7 RNA polymerases: stability of lacZ mRNA inversely correlates with polymerase speed, Proc. Natl. Acad. Sci. U.S.A, vol.92, pp.12250-12254, 1995.

M. De-la-pena, O. J. Kyrieleis, and S. Cusack, Structural insights into the mechanism and evolution of the vaccinia virus mRNA cap N7 methyl-transferase, EMBO J, vol.26, pp.4913-4925, 2007.

S. Dube, J. W. Fisher, and J. S. Powell, Glycosylation at specific sites of erythropoietin is essential for biosynthesis, secretion, and biological function, J. Biol. Chem, vol.263, pp.17516-17521, 1988.

E. Dauty and A. S. Verkman, Actin cytoskeleton as the principal determinant of size-dependent DNA mobility in cytoplasm: a new barrier for non-viral gene delivery, J. Biol. Chem, vol.280, pp.7823-7828, 2005.

D. R. Gallie, The cap and poly(A) tail function synergistically to regulate mRNA translational efficiency, Genes Dev, vol.5, pp.2108-2116, 1991.

R. Parker and H. Song, The enzymes and control of eukaryotic mRNA turnover, Nat. Struct. Mol. Biol, vol.11, pp.121-127, 2004.

D. Cao and R. Parker, Computational modeling of eukaryotic mRNA turnover, RNA, vol.7, pp.1192-1212, 2001.

G. Brawerman, The Role of the poly(A) sequence in mammalian messenger RNA, CRC Crit. Rev. Biochem, vol.10, pp.1-38, 1981.

J. Zhao, L. Hyman, and C. Moore, Formation of mRNA 3' ends in eukaryotes: mechanism, regulation, and interrelationships with other steps in mRNA synthesis. Microbiol, Mol. Biol. Rev, vol.63, pp.405-445, 1999.

E. Wahle, Poly(A) tail length control is caused by termination of processive synthesis, J. Biol. Chem, vol.270, pp.2800-2808, 1995.

M. D. Sheets and M. Wickens, Two phases in the addition of a poly(A) tail, Genes Dev, vol.3, pp.1401-1412, 1989.

J. Peng, E. L. Murray, and D. R. Schoenberg, In vivo and in vitro analysis of poly(A) length effects on mRNA translation, Methods Mol. Biol, vol.419, pp.215-230, 2008.

V. Hornung, J. Ellegast, S. Kim, K. Brzozka, A. Jung et al., ) 5'-Triphosphate RNA is the ligand for RIG-I, Science, vol.314, pp.994-997, 2006.

M. Yoneyama, M. Kikuchi, T. Natsukawa, N. Shinobu, T. Imaizumi et al., The RNA helicase RIG-I has an essential function in double-stranded RNA-induced innate antiviral responses, Nat. Immunol, vol.5, pp.730-737, 2004.

S. Daffis, K. J. Szretter, J. Schriewer, J. Li, S. Youn et al., ) 2'-O methylation of the viral mRNA cap evades host restriction by IFIT family members, Nature, vol.468, pp.452-456, 2010.

J. Mauer, X. Luo, A. Blanjoie, X. Jiao, A. V. Grozhik et al., Reversible methylation of m(6)Am in the 5' cap controls mRNA stability, Nature, vol.541, pp.371-375, 2017.

K. D. Meyer, Y. Saletore, P. Zumbo, O. Elemento, C. E. Mason et al., Comprehensive analysis of mRNA methylation reveals enrichment in 3' UTRs and near stop codons, Cell, vol.149, pp.1635-1646, 2012.

J. E. Squires, H. R. Patel, M. Nousch, T. Sibbritt, D. T. Humphreys et al., Widespread occurrence of 5-methylcytosine in human coding and non-coding RNA, Nucleic Acids Res, vol.40, pp.5023-5033, 2012.

L. Hir, H. Nott, A. Moore, and M. J. , How introns influence and enhance eukaryotic gene expression, Trends Biochem. Sci, vol.28, pp.215-220, 2003.

K. Matsumoto, K. M. Wassarman, and A. P. Wolffe, Nuclear history of a pre-mRNA determines the translational activity of cytoplasmic mRNA, EMBO J, vol.17, pp.2107-2121, 1998.

T. L. Fink, P. J. Klepcyk, S. M. Oette, C. R. Gedeon, S. L. Hyatt et al., Plasmid size up to 20 kbp does not limit effective in vivo lung gene transfer using compacted DNA nanoparticles, Gene Ther, vol.13, pp.1048-1051, 2006.

F. Labat-moleur, A. M. Steffan, C. Brisson, H. Perron, O. Feugeas et al., An electron microscopy study into the mechanism of gene transfer with lipopolyamines, Gene Ther, vol.3, pp.1010-1017, 1996.

R. Tachibana, H. Harashima, Y. Shinohara, and H. Kiwada, Quantitative studies on the nuclear transport of plasmid DNA and gene expression employing nonviral vectors, Adv. Drug Deliv. Rev, vol.52, pp.219-226, 2001.

H. Yin, R. L. Kanasty, A. A. Eltoukhy, A. J. Vegas, J. R. Dorkin et al., Non-viral vectors for gene-based therapy, Nat. Rev. Genet, vol.15, pp.541-555, 2014.

G. D. Schmidt-wolf and I. G. Schmidt-wolf, Non-viral and hybrid vectors in human gene therapy: an update, Trends Mol. Med, vol.9, pp.67-72, 2003.

H. E. Eaton, T. Kobayashi, T. S. Dermody, R. N. Johnston, P. H. Jais et al., African swine fever virus NP868R capping enzyme promotes reovirus rescue during reverse genetics by promoting reovirus protein expression, virion assembly, and RNA incorporation into infectious virions, J. Virol, vol.91, pp.2416-2432, 2017.

M. R. Duncan, S. M. Stanish, and D. C. Cox, Differential sensitivity of normal and transformed human cells to reovirus infection, J. Virol, vol.28, pp.444-449, 1978.

K. A. Mookhtiar, P. S. Peluso, D. K. Muller, J. J. Dunn, and J. E. Coleman, Processivity of T7 RNA polymerase requires the C-terminal Phe882-Ala883-COO-or "foot, Biochemistry, vol.30, pp.6305-6313, 1991.

L. P. Gardner, K. A. Mookhtiar, and J. E. Coleman, Initiation, elongation, and processivity of carboxyl-terminal mutants of T7 RNA polymerase, Biochemistry, vol.36, pp.2908-2918, 1997.