Cloning and expression of a queen pheromone-binding protein in the honeybee: an olfactory-specific, developmentally regulated protein.

Abstract : Odorant-binding proteins (OBPs) are small abundant extracellular proteins thought to participate in perireceptor events of odor-pheromone detection by carrying, deactivating, and/or selecting odor stimuli. The honeybee queen pheromone is known to play a crucial role in colony organization, in addition to drone sex attraction. We identified, for the first time in a social insect, a binding protein called antennal-specific protein 1 (ASP1), which binds at least one of the major queen pheromone components. ASP1 was characterized by cDNA cloning, expression in Pichia pastoris, and pheromone binding. In situ hybridization showed that it is specifically expressed in the auxiliary cell layer of the antennal olfactory sensilla. The ASP1 sequence revealed it as a divergent member of the insect OBP family. The recombinant protein presented the exact characteristics of the native protein, as shown by mass spectrometry, and N-terminal sequencing and exclusion-diffusion chromatography showed that recombinant ASP1 is dimeric. ASP1 interacts with queen pheromone major components, opposite to another putative honeybee OBP, called ASP2. ASP1 biosynthetic accumulation, followed by nondenaturing electrophoresis during development, starts at day 1 before emergence, in concomitance with the functional maturation of olfactory neurons. The isobar ASP1b isoform appears simultaneously to ASP1a in workers, but only at approximately 2 weeks after emergence in drones. Comparison of in vivo and heterologous expressions suggests that the difference between ASP1 isoforms might be because of dimerization, which might play a physiological role in relation with mate attraction.
Complete list of metadatas

Cited literature [52 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-02001719
Contributor : Emmanuelle Berger <>
Submitted on : Thursday, January 31, 2019 - 12:23:49 PM
Last modification on : Thursday, May 16, 2019 - 1:23:50 AM
Long-term archiving on : Wednesday, May 1, 2019 - 4:23:52 PM

File

 Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed until : jamais

Please log in to resquest access to the document

Identifiers

  • HAL Id : inserm-02001719, version 1
  • PUBMED : 10460253

Collections

Citation

Emmanuelle Berger, Loïc Briand, Christine Michard-Vanhée, Valérie Perez, Gérard Arnold, et al.. Cloning and expression of a queen pheromone-binding protein in the honeybee: an olfactory-specific, developmentally regulated protein.. Journal of Neuroscience, Society for Neuroscience, 1999, 19 (17), pp.7468-75. ⟨inserm-02001719⟩

Share

Metrics

Record views

78