Targeted Sequencing of the Mitochondrial Genome of Women at High Risk of Breast Cancer without Detectable Mutations in BRCA1/2

Sophie Blein; Laure Barjhoux; Francesca Damiola; Marie-Gabrielle Dondon; Séverine Eon-Marchais; Morgane Marcou; Olivier Caron; Alain Lortholary; Bruno Buecher; Philippe Vennin; Pascaline Berthet; Catherine Noguès; Christine Lasset; Marion Gauthier-Villars; Sylvie Mazoyer; Dominique Stoppa-Lyonnet; Nadine Andrieu; Gilles Thomas; Olga Sinilnikova; David Cox

Targeted Sequencing of the Mitochondrial Genome of Women at High Risk of Breast Cancer without Detectable Mutations in BRCA1/2

Sophie Blein ¹ Laure Barjhoux ^{2, 1} Francesca Damiola ¹ Marie-Gabrielle Dondon ³ Séverine Eon-Marchais ³ Morgane Marcou ³ Olivier Caron ⁴ Alain Lortholary ⁵ Bruno Buecher ⁶ Philippe Vennin ⁷ Pascaline Berthet ^{8, 9} Catherine Noguès ¹⁰ Christine Lasset ¹¹ Marion Gauthier-Villars ⁴ Sylvie Mazoyer ¹ Dominique Stoppa-Lyonnet ^{12, 13} Nadine Andrieu ³ Gilles Thomas ¹⁴ Olga Sinilnikova ^{1, 2} David Cox ^{1, *}

* Corresponding author

1 CRCL - Centre de Recherche en Cancérologie de Lyon

2 Unité Mixte de Génétique Constitutionnelle des Cancers Fréquents

3 Cancer et génôme: Bioinformatique, biostatistiques et épidémiologie d'un système complexe

4 Onco-génétique

Département de médecine oncologique [Gustave Roussy]

5 Centre Catherine-de-Sienne [Nantes]

6 Institut Curie

7 CRLCC Oscar Lambret

8 Consultation d'Oncogénétique

9 CRLC François Baclesse - Centre Régional de Lutte contre le Cancer François Baclesse

10 CRLCC René Huguenin

11 Centre Léon Bérard [Lyon]

12 Service de Génétique Oncologique

13 U830 - Unité de génétique et biologie des cancers

14 Université de Lyon

Abstract : Breast Cancer is a complex multifactorial disease for which high-penetrance mutations have been identified. Approaches used to date have identified genomic features explaining about 50% of breast cancer heritability. A number of low-to medium penetrance alleles (per-allele odds ratio < 1.5 and 4.0, respectively) have been identified, suggesting that the remaining heritability is likely to be explained by the cumulative effect of such alleles and/or by rare high-penetrance alleles. Relatively few studies have specifically explored the mito-chondrial genome for variants potentially implicated in breast cancer risk. For these reasons , we propose an exploration of the variability of the mitochondrial genome in individuals diagnosed with breast cancer, having a positive breast cancer family history but testing negative for BRCA1/2 pathogenic mutations. We sequenced the mitochondrial genome of 436 index breast cancer cases from the GENESIS study. As expected, no pathogenic genomic pattern common to the 436 women included in our study was observed. The mitochondrial genes MT-ATP6 and MT-CYB were observed to carry the highest number of variants in the study. The proteins encoded by these genes are involved in the structure of the PLOS ONE |

Document type :

Journal articles

Domain :

Life Sciences [q-bio] / Cancer

Life Sciences [q-bio] / Genetics

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https://www.hal.inserm.fr/inserm-01991386
Contributor : Claire Lissalde <>
Submitted on : Wednesday, January 23, 2019 - 6:35:24 PM
Last modification on : Wednesday, December 4, 2019 - 9:48:02 AM

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Targeted Sequencing of the Mitochondrial Genome of Women at High Risk of Breast Cancer without Detectable Mutations in BRCA1/2

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Targeted Sequencing of the Mitochondrial Genome of Women at High Risk of Breast Cancer without Detectable Mutations in BRCA1/2

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