Reduced ex vivo susceptibility of Plasmodium falciparum after oral artemether–lumefantrine treatment in Mali - Archive ouverte HAL Access content directly
Journal Articles Malaria Journal Year : 2017

Reduced ex vivo susceptibility of Plasmodium falciparum after oral artemether–lumefantrine treatment in Mali

(1, 2) , (1) , (1) , (1) , (1) , (1) , (1) , (1) , (1) , (1) , (1) , (1) , (1) , (1) , (1) , (1) , (1)
1
2

Abstract

Background: Artemisinin-based combination therapy is the recommended first-line treatment for uncomplicated falciparum malaria worldwide. However, recent studies conducted in Mali showed an increased frequency of recurrent parasitaemia following artemether-lumefantrine (AL) treatment. Methods: Study samples were collected during a large WANECAM study. Ex-vivo Plasmodium falciparum sensitivity to artemether and lumefantrine was assessed using the tritiated hypoxanthine-based assay. The prevalence of molecular markers of anti-malarial drug resistance (pfcrt K76T, pfmdr1 N86Y and K13-propeller) were measured by PCR and/or sequencing. Results: Overall 61 samples were successfully analysed in ex vivo studies. Mean IC 50 s increased significantly between baseline and recurrent parasites for both artemether (1.6 nM vs 3.2 nM, p < 0.001) and lumefantrine (1.4 nM vs 3.4 nM, p = 0.004). Wild type Pfmdr1 N86 allele was selected after treatment (71 vs 91%, 112 of 158 vs 95 of 105, p < 0.001) but not the wild type pfcrt K76 variant (23.5 vs 24.8%, 40 of 170 vs 26 of 105, p = 0.9). Three non-synonymous K13-propeller SNPs (A522C, A578S, and G638R) were found with allele frequencies <2%. Conclusion: Malian postAL P. falciparum isolates were less susceptible to artemether and lumefantrine than baseline isolates.
Fichier principal
Vignette du fichier
12936_2017_Article_1700.pdf (953.67 Ko) Télécharger le fichier
Origin : Publisher files allowed on an open archive
Loading...

Dates and versions

inserm-01980382 , version 1 (14-01-2019)

Identifiers

Cite

Souleymane Dama, Hamidou Niangaly, Amed Ouattara, Issaka Sagara, Sekou Sissoko, et al.. Reduced ex vivo susceptibility of Plasmodium falciparum after oral artemether–lumefantrine treatment in Mali. Malaria Journal, 2017, 16 (1), ⟨10.1186/s12936-017-1700-8⟩. ⟨inserm-01980382⟩

Collections

INSERM
61 View
124 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More