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International external quality assurance of JAK2 V617F quantification

Julia Asp 1, * Vibe Skov 2 Beatriz Bellosillo 3 Thomas Kristensen 4 Eric Lippert 5 Frank Dicker 6 Jiri Schwarz 7 Marzena Wojtaszewska 8 Lars Palmqvist 1 Susanna Akiki 9 Anni Aggerholm 10 Morten Tolstrup Andersen 11 François Girodon 12, 13 Lasse Kjær 2 Elisabeth Oppliger Leibundgut 14 Alessandro Pancrazzi 15 Marta Vorland 16 Hajnalka Andrikovics 17 Robert Kralovics 18, 19 Bruno Cassinat 20 Margarida Coucelo 21 Aleksandar Eftimov 22 Karl Haslam 23 Rajko Kusec 24 Dorota Link-Lenczowska 25 Laurence Lode 26 Karolina Matiakowska 27 Dina Naguib 28 Filippo Navaglia 29 Guy Wayne Novotny 30 Melanie J Percy 31 Andrey Sudarikov 32 Sylvie Hermouet 33, 34, * Niels Pallisgaard 2 
* Corresponding author
33 CRCINA-ÉQUIPE 16 - Molecular Mechanisms of Chronic Inflammation in Hematological Diseases
CRCINA - Centre de Recherche en Cancérologie et Immunologie Nantes-Angers
Abstract : External quality assurance (EQA) programs are vital to ensure high quality and standardized results in molecular diagnostics. It is important that EQA for quantitative analysis takes into account the variation in methodology. Results cannot be expected to be more accurate than limits of the technology used, and it is essential to recognize factors causing substantial outlier results. The present study aimed to identify parameters of specific importance for JAK2 V617F quantification by quantitative PCR, using different starting materials, assays, and technical platforms. Sixteen samples were issued to participating laboratories in two EQA rounds. In the first round, 19 laboratories from 11 European countries analyzing JAK2 V617F as part of their routine diagnostics returned results from in-house assays. In the second round, 25 laboratories from 17 countries participated. Despite variations in starting material, assay setup and instrumentation the laboratories were generally well aligned in the EQA program. However, EQA based on a single technology appears to be a valuable tool to achieve standardization of the quantification of JAK2 V617F allelic burden.
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Julia Asp, Vibe Skov, Beatriz Bellosillo, Thomas Kristensen, Eric Lippert, et al.. International external quality assurance of JAK2 V617F quantification. Annals of Hematology, Springer Verlag, 2018, Epub ahead of print. ⟨10.1007/s00277-018-3570-8⟩. ⟨inserm-01968802⟩



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