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Journal articles
Conventional and Neo-Antigenic Peptides Presented by β Cells Are Targeted by Circulating Naïve CD8+ T Cells in Type 1 Diabetic and Healthy Donors
Sergio Gonzalez-Duque
1, 2, 3
Marie Eliane Azoury
1, 2, 4
Maikel Colli
5
Georgia Afonso
1, 2, 4
Jean-Valery Turatsinze
5
Laura Nigi
6
Ana Inés Lalanne
1, 2, 4
Guido Sebastiani
6
Alexia Carré
1, 2, 4
Sheena Pinto
7
Slobodan Culina
1, 2, 4
Noémie Corcos
1, 2, 4
Marco Bugliani
8
Marc Marchetti
8
Mathieu Armanet
9
Marc Diedisheim
1, 2, 10, 4, 11
Bruno Kyewski
7
Lars Steinmetz
12, 13
Søren Buus
14
Sylvaine You
2, 1, 4
Daniele Dubois-Laforgue
2, 1, 10, 4, 11
Etienne Larger
2, 1, 10, 4, 11
Jean-Paul Beressi
15
Graziella Bruno
16
Francesco Dotta
6
Raphael Scharfmann
1, 2, 4
Decio Eizirik
5
Yann Verdier
17
Joelle Vinh
17
Roberto Mallone
1, 2, 10, 4, 11, *
*
Corresponding author
Sergio Gonzalez-Duque 1, 2, 3
Author
Marie Eliane Azoury 1, 2, 4
Author
Maikel Colli 5
Author
Georgia Afonso 1, 2, 4
Author
Jean-Valery Turatsinze 5
Author
Laura Nigi 6
Author
Ana Inés Lalanne 1, 2, 4
Author
Guido Sebastiani 6
Author
Alexia Carré 1, 2, 4
Author
Sheena Pinto 7
Author
Slobodan Culina 1, 2, 4
Author
Noémie Corcos 1, 2, 4
Author
Marco Bugliani 8
Author
Marc Marchetti 8
Author
Mathieu Armanet 9
Author
Marc Diedisheim 1, 2, 10, 4, 11
Author
Bruno Kyewski 7
Author
Lars Steinmetz 12, 13
Author
Søren Buus 14
Author
Sylvaine You 2, 1, 4
Author
Daniele Dubois-Laforgue 2, 1, 10, 4, 11
Author
Etienne Larger 2, 1, 10, 4, 11
Author
Jean-Paul Beressi 15
Author
Graziella Bruno 16
Author
Francesco Dotta 6
Author
Raphael Scharfmann 1, 2, 4
Author
Decio Eizirik 5
Author
Yann Verdier 17
Author
Joelle Vinh 17
Author IdHAL : joellevinh ResearcherId : http://www.researcherid.com/rid/K-1347-2015 ORCID : https://orcid.org/0000-0001-7184-2668
Roberto Mallone 1, 2, 10, 4, 11
Correspondent author
1
IC UM3 (UMR 8104 / U1016) - Institut Cochin (22 rue Méchain, 75014 Paris - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U1016 (101, rue de Tolbiac, 75013 Paris - France)
- UPD5 - Université Paris Descartes - Paris 5 : UM 3 (12, rue de l'École de Médecine - 75270 Paris cedex 06 - France)
- CNRS - Centre National de la Recherche Scientifique : UMR 8104 (France)
2
USPC - Université Sorbonne Paris Cité (Université Sorbonne Paris Cité | 100-104, avenue de France | 75013 Paris - France)
3
ESPCI Paris - Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (10 rue Vauquelin, 75231 Paris cedex 05 - France)
- PSL - Université Paris sciences et lettres (60 rue Mazarine 75006 Paris - France)
4
UPD5 - Université Paris Descartes - Paris 5 (12, rue de l'École de Médecine - 75270 Paris cedex 06 - France)
5
Medical Faculty - Center for Diabetes Research and Welbio [Brussels, Belgium] (Belgium)
- ULB - Université libre de Bruxelles (Avenue Franklin Roosevelt 50 - 1050 Bruxelles - Belgium)
7
Division of Developmental Immunology [Heidelberg, Germany] (Germany)
- DKFZ - German Cancer Research Center - Deutsches Krebsforschungszentrum [Heidelberg] (German Cancer Research Center Im Neuenheimer Feld 280 D-69120 Heidelberg - Germany)
8
Department of Clinical and Experimental Medicine [Pisa, Italy] (Italy)
- University of Pisa [Italy] (Italy)
9
Cell Therapy Unit [AP-HP Hôpital Saint-Louis] (1 Avenue Claude Vellefaux 75010 Paris - France)
- AP-HP - Assistance publique - Hôpitaux de Paris (AP-HP) (France)
- UPD7 - Université Paris Diderot - Paris 7 (5 rue Thomas-Mann - 75205 Paris cedex 13 - France)
- Groupe Hospitalier Saint Louis - Lariboisière - Fernand Widal [Paris] (200 rue du Faubourg Saint-Denis 75475 Paris (Nord) Cedex 10 - France)
10
Service de diabétologie [CHU Cochin] (27 Rue du Faubourg Saint-Jacques, 75014 Paris - France)
- AP-HP - Assistance publique - Hôpitaux de Paris (AP-HP) (France)
- CHU Cochin [AP-HP] (27 Rue du Faubourg Saint-Jacques 75014 Paris - France)
11
CHU Cochin [AP-HP] (27 Rue du Faubourg Saint-Jacques 75014 Paris - France)
12
School of Medicine - Department of Genetics and Stanford Genome Technology Center [Stanford, CA, USA] (United States)
- Stanford University [Stanford] (450 Serra Mall, Stanford, CA 94305-2004 - United States)
13
Genome Biology Unit [Heidelberg, Germany] (Germany)
- European Molecular Biology Laboratory [Hinxton] (United Kingdom)
14
Department of Immunology and Microbiology [Copenhagen] (Panum
Blegdamsvej 3B
DK-2200 Copenhagen N
Denmark - Denmark)
- Faculty of Health and Medical Sciences (Blegdamsvej 3B
DK-2200 Copenhagen N
Denmark - Denmark)
- KU - University of Copenhagen = Københavns Universitet (Nørregade 10, 1165 København, Danemark - Denmark)
15
Service de Diabétologie [CH Versailles André Mignot, Le Chesnay] (France)
- CHV - Centre Hospitalier de Versailles André Mignot (Versailles, Trappes, Andrésy et Bois d'Arcy, Chesnay - France)
16
Department of Medical Sciences [Turin, Italy] (Italy)
- University of Turin (Via Verdi, 8 - 10124 Turin - Italy)
17
SMBP - Laboratoire de Spectrométrie de Masse Biologique & Protéomique (École Supérieure de Physique et de Chimie Industrielles de la Ville de Paris 10, rue Vauquelin - 75231 Paris Cedex 05 - France)
- ESPCI Paris - Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (10 rue Vauquelin, 75231 Paris cedex 05 - France)
- PSL - Université Paris sciences et lettres (60 rue Mazarine 75006 Paris - France)
- CNRS - Centre National de la Recherche Scientifique : USR3149 (France)
Abstract : Although CD8+ T-cell-mediated autoimmune β cell destruction occurs in type 1 diabetes (T1D), the target epitopes processed and presented by β cells are unknown. To identify them, we combined peptidomics and transcriptomics strategies. Inflammatory cytokines increased peptide presentation in vitro, paralleling upregulation of human leukocyte antigen (HLA) class I expression. Peptide sources featured several insulin granule proteins and all known β cell antigens, barring islet-specific glucose-6-phosphatase catalytic subunit-related protein. Preproinsulin yielded HLA-A2-restricted epitopes previously described. Secretogranin V and its mRNA splice isoform SCG5-009, proconvertase-2, urocortin-3, the insulin gene enhancer protein ISL-1, and an islet amyloid polypeptide transpeptidation product emerged as antigens processed into HLA-A2-restricted epitopes, which, as those already described, were recognized by circulating naive CD8+ T cells in T1D and healthy donors and by pancreas-infiltrating cells in T1D donors. This peptidome opens new avenues to understand antigen processing by β cells and for the development of T cell biomarkers and tolerogenic vaccination strategies.
Document type :
Journal articles
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Complete list of metadatas
https://www.hal.inserm.fr/inserm-01918834
Contributor : Roberto Mallone <>
Submitted on : Monday, November 12, 2018 - 12:46:10 AM
Last modification on : Saturday, September 26, 2020 - 11:44:12 PM
Long-term archiving on: : Wednesday, February 13, 2019 - 12:44:34 PM
Contributor : Roberto Mallone <>
Submitted on : Monday, November 12, 2018 - 12:46:10 AM
Last modification on : Saturday, September 26, 2020 - 11:44:12 PM
Long-term archiving on: : Wednesday, February 13, 2019 - 12:44:34 PM
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Identifiers
- HAL Id : inserm-01918834, version 1
- DOI : 10.1016/j.cmet.2018.07.007
- PUBMED : 30078552
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CNRS | USPC | PARISTECH | UNIV-PARIS5 | UNIV-PARIS7 | UNIV-PARIS | UP-SANTE | PSL | ESPCI-PSL | ESPCI
Citation
Sergio Gonzalez-Duque, Marie Eliane Azoury, Maikel Colli, Georgia Afonso, Jean-Valery Turatsinze, et al.. Conventional and Neo-Antigenic Peptides Presented by β Cells Are Targeted by Circulating Naïve CD8+ T Cells in Type 1 Diabetic and Healthy Donors. Cell Metabolism, Elsevier, 2018, 28 (6), pp.946-960. ⟨10.1016/j.cmet.2018.07.007⟩. ⟨inserm-01918834⟩
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