Skip to Main content Skip to Navigation
Journal articles

The recombination mediator RAD51D promotes geminiviral infection

Abstract : To study a possible role for homologous recombination in geminivirus replication, we challenged Arabidopsis recombination gene knockouts by Euphorbia yellow mosaic virus infection. Our results show that the RAD51 paralog RAD51D, rather than RAD51 itself, promotes viral replication at early stages of infection. Blot hybridization analyses of replicative intermediates using one- and two-dimensional gels and deep sequencing point to an unexpected facet of recombination-dependent replication, the repair by single-strand annealing (SSA) during complementary strand replication. A significant decrease of both intramolecular, yielding defective DNAs and intermolecular recombinant molecules between the two geminiviral DNA components (A, B) were observed in the absence of RAD51D. By contrast, DNA A and B reacted differentially with the generation of inversions. A model to implicate single-strand annealing recombination in geminiviral recombination-dependent replication is proposed.
Document type :
Journal articles
Complete list of metadata

Cited literature [99 references]  Display  Hide  Download
Contributor : Charles White Connect in order to contact the contributor
Submitted on : Thursday, October 25, 2018 - 1:55:39 PM
Last modification on : Tuesday, April 20, 2021 - 11:22:02 AM
Long-term archiving on: : Saturday, January 26, 2019 - 2:16:52 PM


 Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed until : jamais

Please log in to resquest access to the document




Kathrin Richter, Heϊdi Serra, Charles I. White, Holger Jeske. The recombination mediator RAD51D promotes geminiviral infection. Virology, Elsevier, 2016, 493, pp.113 - 127. ⟨10.1016/j.virol.2016.03.014⟩. ⟨inserm-01904883⟩



Record views