Skip to Main content Skip to Navigation
Journal articles

RAD54 forms DNA repair foci in response to DNA damage in living plant cells

Abstract : Plants have various defense mechanisms against environmental stresses that induce DNA damage. Genetic and biochemical analyses have revealed the sensing and signaling of DNA damage, but little is known about subnuclear dynamics in response to DNA damage in living plant cells. Here, we observed that the chromatin remodeling factor RAD54, which is involved in DNA repair via the homologous recombination pathway, formed subnuclear foci (termed RAD54 foci) in Arabidopsis thaliana after induction of DNA double-strand breaks. The appearance of RAD54 foci was dependent on the ATAXIA-TELANGIECTASIA MUTATED-SUPPRESSOR OF GAMMA RESPONSE 1 pathway, and RAD54 foci were co-localized with γH2AX signals. Laser irradiation of a subnuclear area demonstrated that in living cells RAD54 was specifically accumulated at the damaged site. In addition, the formation of RAD54 foci showed specificity for cell type and region. We conclude that RAD54 foci correspond to DNA repair foci in A. thaliana.
Document type :
Journal articles
Complete list of metadata

Cited literature [45 references]  Display  Hide  Download
Contributor : Charles White Connect in order to contact the contributor
Submitted on : Thursday, October 25, 2018 - 1:42:57 PM
Last modification on : Tuesday, February 23, 2021 - 11:34:04 AM
Long-term archiving on: : Saturday, January 26, 2019 - 2:30:11 PM


 Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed until : jamais

Please log in to resquest access to the document




Takeshi Hirakawa, Junko Hasegawa, Charles I. White, Sachihiro Matsunaga. RAD54 forms DNA repair foci in response to DNA damage in living plant cells. Plant Journal, Wiley, 2017, 90 (2), pp.372 - 382. ⟨10.1111/tpj.13499⟩. ⟨inserm-01904874⟩



Record views