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Journal articles
RAD54 forms DNA repair foci in response to DNA damage in living plant cells
Abstract : Plants have various defense mechanisms against environmental stresses that induce DNA damage. Genetic and biochemical analyses have revealed the sensing and signaling of DNA damage, but little is known about subnuclear dynamics in response to DNA damage in living plant cells. Here, we observed that the chromatin remodeling factor RAD54, which is involved in DNA repair via the homologous recombination pathway, formed subnuclear foci (termed RAD54 foci) in Arabidopsis thaliana after induction of DNA double-strand breaks. The appearance of RAD54 foci was dependent on the ATAXIA-TELANGIECTASIA MUTATED-SUPPRESSOR OF GAMMA RESPONSE 1 pathway, and RAD54 foci were co-localized with γH2AX signals. Laser irradiation of a subnuclear area demonstrated that in living cells RAD54 was specifically accumulated at the damaged site. In addition, the formation of RAD54 foci showed specificity for cell type and region. We conclude that RAD54 foci correspond to DNA repair foci in A. thaliana.
Cited literature [45 references]
https://www.hal.inserm.fr/inserm-01904874
Contributor : Charles White Connect in order to contact the contributor
Submitted on : Thursday, October 25, 2018 - 1:42:57 PM
Last modification on : Tuesday, February 23, 2021 - 11:34:04 AM
Long-term archiving on: : Saturday, January 26, 2019 - 2:30:11 PM
Contributor : Charles White Connect in order to contact the contributor
Submitted on : Thursday, October 25, 2018 - 1:42:57 PM
Last modification on : Tuesday, February 23, 2021 - 11:34:04 AM
Long-term archiving on: : Saturday, January 26, 2019 - 2:30:11 PM