Analysis of the impact of the absence of RAD51 strand exchange activity in Arabidopsis meiosis

Abstract : The ploidy of eukaryote gametes must be halved to avoid doubling of numbers of chromosomes with each generation and this is carried out by meiosis, a specialized cell division in which a single chromosomal replication phase is followed by two successive nuclear divisions. With some exceptions, programmed recombination ensures the proper pairing and distribution of homologous pairs of chromosomes in meiosis and recombination defects thus lead to sterility. Two highly related recombinases are required to catalyse the key strand-invasion step of meiotic recombination and it is the meiosis-specific DMC1 which is generally believed to catalyse the essential non-sister chromatid crossing-over, with RAD51 catalysing sister-chromatid and non-cross-over events. Recent work in yeast and plants has however shown that in the absence of RAD51 strand-exchange activity, DMC1 is able to repair all meiotic DNA breaks and surprisingly, that this does not appear to affect numbers of meiotic cross-overs. In this work we confirm and extend this conclusion. Given that more than 95% of meiotic homologous recombination in Arabidopsis does not result in inter-homologue crossovers, Arabidopsis is a particularly sensitive model for testing the relative importance of the two proteins-even minor effects on the non-crossover event population should produce detectable effects on crossing-over. Although the presence of RAD51 protein provides essential support for the action of DMC1, our results show no significant effect of the absence of RAD51 strand-exchange activity on meiotic crossing-over rates or patterns in different chromosomal regions or across the whole genome of Arabidopsis, strongly supporting the argument that DMC1 catalyses repair of all meiotic DNA breaks, not only non-sister cross-overs.
Document type :
Journal articles
Complete list of metadatas

Cited literature [67 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-01904871
Contributor : Charles White <>
Submitted on : Thursday, October 25, 2018 - 1:37:52 PM
Last modification on : Tuesday, November 5, 2019 - 12:34:10 PM
Long-term archiving on : Saturday, January 26, 2019 - 1:57:29 PM

File

journal.pone.0183006.pdf
Publication funded by an institution

Identifiers

Collections

Citation

Gunjita Singh, Olivier da Ines, Maria Eugenia Gallego, Charles White. Analysis of the impact of the absence of RAD51 strand exchange activity in Arabidopsis meiosis. PLoS ONE, Public Library of Science, 2017, 12 (8), pp.e0183006. ⟨10.1371/journal.pone.0183006⟩. ⟨inserm-01904871⟩

Share

Metrics

Record views

77

Files downloads

172