New insights into the tetraspanin Tspan5 using novel monoclonal antibodies

Abstract : Tspan5 is a member of a subgroup of tetraspanins referred to as TspanC8. These tetraspanins directly interact with the metalloprotease ADAM10, regulate its exit from the endoplasmic reticulum and subsequent trafficking, and differentially regulate its ability to cleave various substrates and activate Notch signaling. The study of Tspan5 has been limited by the lack of good antibodies. This study provides new insights into Tspan5 using new monoclonal antibodies (mAbs), including two mAbs recognizing both Tspan5 and the highly similar tetraspanin Tspan17. Using these mAbs, we show that endogenous Tspan5 associates with ADAM10 in human cell lines and in mouse tissues where it is the most abundant, such as the brain, the lung, the kidney, or the intestine. We also uncover two TspanC8-specific motifs in the large extracellular domain of Tspan5 that are important for ADAM10 interaction and exit from the endoplasmic reticulum. One of the anti-Tspan5 mAbs does not recognize Tspan5 associated with ADAM10, providing a convenient way to measure the fraction of Tspan5 not associated with ADAM10. This fraction is minor in the cell lines tested, and it increases upon transfection of cells with TspanC8 tetraspanins such as Tspan15 or Tspan33 that inhibit Notch signaling. Finally, two antibodies inhibit ligand-induced Notch signaling, and this effect is stronger in cells depleted of the TspanC8 tetraspanin Tspan14, further indicating that Tspan5 and Tspan14 can compensate for each other in Notch signaling.
Document type :
Journal articles
Complete list of metadatas

Cited literature [34 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-01904372
Contributor : Eric Rubinstein <>
Submitted on : Wednesday, October 24, 2018 - 6:37:53 PM
Last modification on : Friday, November 8, 2019 - 10:48:04 AM
Long-term archiving on : Friday, January 25, 2019 - 3:41:54 PM

File

Saint-Pol JBC 2017.pdf
Publisher files allowed on an open archive

Identifiers

Citation

Julien Saint-Pol, Martine Billard, Emmanuel Dornier, Etienne Eschenbrenner, Lydia Danglot, et al.. New insights into the tetraspanin Tspan5 using novel monoclonal antibodies. Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2017, 292 (23), pp.9551 - 9566. ⟨10.1074/jbc.M116.765669⟩. ⟨inserm-01904372⟩

Share

Metrics

Record views

125

Files downloads

128