Measurement of serum IgG4 levels by a competitive immunoenzymatic assay with monoclonal antibodies.

Pierre Aucouturier 1, * Françoise Danon 2 Maryvonne Daveau 3 Bernard Guillou 4 Alfred Sabbah 5 Jacques Besson 6 Jean-Louis Preud'Homme 1
* Corresponding author
1 Laboratoire d'lmmunologie et Immunopathologie [CHRU La Milétrie, Poitiers]
2 INSERM U108 - Laboratoire d'lmmunochimie et Immunopathologie [AP-HP Hôpital Saint-Louis]
3 U78 INSERM - Unité de Recherches sur la Génétique des Protéines Humaines [Bois-Guillaume]
4 LA 191 CNRS - Laboratoire de Thermique de I'ENSMA [Poitiers]
5 Laboratoire d'Immunoallergologie [CHUR Angers]
6 Faculté de Médecine - Département de Statistique [CHRU Poitiers]
Abstract : A competitive indirect ELISA is described for the measurement of IgG4 levels. It uses a monoclonal anti-subclass and antibody and purified monoclonal IgG4 as standards. This method is sensitive and reproducible and more accurate than hemagglutination inhibition and radial immunodiffusion. Serum IgG4 levels in 173 normal adults were less than 0.01-2.1 mg/ml (mean 0.30 mg/ml) in women and less than 0.01-1.87 mg/ml (mean 0.465 mg/ml) in men.
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  • HAL Id : inserm-01899792, version 1
  • PUBMED : 6438233

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Pierre Aucouturier, Françoise Danon, Maryvonne Daveau, Bernard Guillou, Alfred Sabbah, et al.. Measurement of serum IgG4 levels by a competitive immunoenzymatic assay with monoclonal antibodies.. Journal of Immunological Methods, Elsevier, 1984, 74 (1), pp.151-62. ⟨inserm-01899792⟩

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