In situ photochemical crosslinking of hydrogel membrane for Guided Tissue Regeneration

Abstract : tObjective. Periodontitis is an inflammatory disease that destroys the tooth-supportingattachment apparatus. Guided tissue regeneration (GTR) is a technique based on a bar-rier membrane designed to prevent wound space colonization by gingival cells. This studyexamined a new formulation composed of two polymers that could be photochemicallycross-linked in situ into an interpenetrated polymer network (IPN) forming a hydrogel mem-brane.Methods. We synthetized and characterized silanized hydroxypropyl methylcellulose (Si-HPMC) for its cell barrier properties and methacrylated carboxymethyl chitosan (MA-CMCS)for its degradable backbone to use in IPN. Hydrogel membranes were cross-linked usingriboflavin photoinitiator and a dentistry visible light lamp. The biomaterial’s physicochem-ical and mechanical properties were determined. Hydrogel membrane degradation wasevaluated in lysozyme. Cytocompatibility was estimated by neutral red uptake. The cell bar-rier property was studied culturing human primary gingival fibroblasts or human gingivalexplants on membrane and analyzed with confocal microscopy and histological staining.Results. The IPN hydrogel membrane was obtained after 120 s of irradiation. The IPN showeda synergistic increase in Young moduli compared with the single networks. The CMCSaddition in IPN allows a progressive weight loss compared to each polymer network. Cyto-compatibility was confirmed by neutral red assay. Human cell invasion was prevented byhydrogel membranes and histological sections revealed that the biomaterial exhibited abarrier effect in contact with soft gingival tissue.
Complete list of metadatas

Cited literature [62 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-01896996
Contributor : Valérie Pecqueret <>
Submitted on : Tuesday, October 16, 2018 - 4:58:01 PM
Last modification on : Thursday, July 11, 2019 - 11:17:28 AM
Long-term archiving on: Thursday, January 17, 2019 - 3:02:35 PM

File

 Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed until : jamais

Please log in to resquest access to the document

Identifiers

Collections

Citation

Pauline Marie Chichiricco, Raphaël Riva, Jean-Michel Thomassin, Julie Lesoeur, Xavier Struillou, et al.. In situ photochemical crosslinking of hydrogel membrane for Guided Tissue Regeneration. Dental Materials, Elsevier, 2018, ⟨10.1016/j.dental.2018.09.017⟩. ⟨inserm-01896996⟩

Share

Metrics

Record views

138