Chromophore-Assisted Light Inactivation of the V-ATPase V0c Subunit Inhibits Neurotransmitter Release Downstream of Synaptic Vesicle Acidification

Sylvain Rama 1 Norah Boumedine-Guignon 1 Marion Sangiardi 1 Fahamoe Youssouf 1 Yves Maulet 1 Christian Levêque 1 Maya Belghazi 2 Michael Seagar 1 Dominique Debanne 1 Oussama El Far 1, *
* Corresponding author
1 UNIS - Inserm U1072 - Unité de Neurobiologie des canaux Ioniques et de la Synapse
2 INP - Institut de neurophysiopathologie
Abstract : Synaptic vesicle proton V-ATPase is an essential component in synaptic vesicle function. Active acidification of synaptic vesicles, triggered by the V-ATPase, is necessary for neurotransmitter storage. Independently from its proton transport activity, an additional important function of the membrane-embedded sector of the V-ATPase has been uncovered over recent years. Subunits a and c of the membrane sector of this multi-molecular complex have been shown to interact with SNARE proteins and to be involved in modulating neurotransmitter release. The c-subunit interacts with the v-SNARE VAMP2 and facilitates neurotransmission. In this study, we used chromophore-assisted light inactivation and monitored the consequences on neuro-transmission on line in CA3 pyramidal neurons. We show that V-ATPase c-subunit V0c is a key element in modulating neurotransmission and that its specific inactivation rapidly inhibited neurotransmission.
Keywords : GeneGun Keywords V-ATPase V-ATPase Chromophore-assisted light inactivation (CALI) GeneGun Hippocampal organotypic slice culture Neurons Neurotransmission
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Submitted on : Friday, September 21, 2018 - 2:18:36 PM
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Sylvain Rama, Norah Boumedine-Guignon, Marion Sangiardi, Fahamoe Youssouf, Yves Maulet, et al.. Chromophore-Assisted Light Inactivation of the V-ATPase V0c Subunit Inhibits Neurotransmitter Release Downstream of Synaptic Vesicle Acidification. Molecular Neurobiology, Humana Press, 2018, 56 (5), pp.3591-3602. ⟨10.1007/s12035-018-1324-1⟩. ⟨inserm-01878772⟩

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