Infected splenic dendritic cells are sufficient for prion transmission to the CNS in mouse scrapie

Abstract : Transmissible spongiform encephalopathies display long incubation periods at the beginning of which the titer of infectious agents (prions) increases in peripheral lymphoid organs. This "replication" leads to a progressive invasion of the CNS. Follicular dendritic cells appear to support prion replication in lymphoid follicles. However, the subsequent steps of neuroinvasion remain obscure. CD11c(+) dendritic cells, an unrelated cell type, are candidate vectors for prion propagation. We found a high infectivity titer in splenic dendritic cells from prion-infected mice, suggesting that dendritic cells carry infection. To test this hypothesis, we injected RAG-1(0/0) mice intravenously with live spleen cell subsets from scrapie-infected donors. Injection of infected dendritic cells induced scrapie without accumulation of prions in the spleen. These results suggest that CD11c(+) dendritic cells can propagate prions from the periphery to the CNS in the absence of any additional lymphoid element.
Complete list of metadatas

Cited literature [24 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-01876604
Contributor : Pierre Aucouturier <>
Submitted on : Tuesday, September 18, 2018 - 3:40:16 PM
Last modification on : Tuesday, August 20, 2019 - 4:34:05 PM

File

articleJCI2001.pdf
Files produced by the author(s)

Identifiers

Collections

Citation

Pierre Aucouturier, Frederic Geissmann, Diane Damotte, Gabriela Saborio, Harry Meeker, et al.. Infected splenic dendritic cells are sufficient for prion transmission to the CNS in mouse scrapie. Journal of Clinical Investigation, American Society for Clinical Investigation, 2001, 108 (5), pp.703 - 708. ⟨10.1172/jci200113155⟩. ⟨inserm-01876604⟩

Share

Metrics

Record views

227

Files downloads

108