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Infected splenic dendritic cells are sufficient for prion transmission to the CNS in mouse scrapie

Abstract : Transmissible spongiform encephalopathies display long incubation periods at the beginning of which the titer of infectious agents (prions) increases in peripheral lymphoid organs. This "replication" leads to a progressive invasion of the CNS. Follicular dendritic cells appear to support prion replication in lymphoid follicles. However, the subsequent steps of neuroinvasion remain obscure. CD11c(+) dendritic cells, an unrelated cell type, are candidate vectors for prion propagation. We found a high infectivity titer in splenic dendritic cells from prion-infected mice, suggesting that dendritic cells carry infection. To test this hypothesis, we injected RAG-1(0/0) mice intravenously with live spleen cell subsets from scrapie-infected donors. Injection of infected dendritic cells induced scrapie without accumulation of prions in the spleen. These results suggest that CD11c(+) dendritic cells can propagate prions from the periphery to the CNS in the absence of any additional lymphoid element.
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Pierre Aucouturier, Frederic Geissmann, Diane Damotte, Gabriela P Saborio, Harry C Meeker, et al.. Infected splenic dendritic cells are sufficient for prion transmission to the CNS in mouse scrapie. Journal of Clinical Investigation, American Society for Clinical Investigation, 2001, 108 (5), pp.703 - 708. ⟨10.1172/jci200113155⟩. ⟨inserm-01876604⟩



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