Skip to Main content Skip to Navigation
Journal articles

In vitro cytotoxic effects of DEHP-alternative plasticizers and their primary metabolites on a L929 cell line

Abstract : Phthalic acid esters have been widely used to improve the plasticity of PVC medical devices. They carry a high exposure risk for both humans and the environment in clinical situations. Our study focuses on the cytotoxicity of alternative plasticizers. Postulated primary metabolites were synthesized, not being commercially available. Cytotoxicity assays were performed on L929 murine cells according to the ISO-EN 10993-5 standard design for the biocompatibility of medical devices. The tested concentrations of plasticizers (0.01, 0.05 and 0.1 mg/ml) covered the range likely to be found in biological fluids coming into direct contact with the medical devices. DEHP, DINP and DINCH were cytotoxic at the highest concentration (0.1 mg/ml) for 7 days of exposure. Their corresponding metabolites were found to be more cytotoxic, for the same concentration. By contrast, TOTM and its corresponding metabolite MOTM were not found to be cytotoxic. DEHA showed no cytotoxicity, but its corresponding monoester (MEHA) produced a cytotoxic effect at 0.05 mg/ml. In clinical situations, medical devices can release plasticizers, which can come into contact with patients. In vivo, the plasticizers are quickly transformed into primary metabolites. It is therefore important to measure the effects of both the plasticizers and their corresponding metabolites. Standard first-line cytotoxicity assays should be performed to ensure biocompatibility.
Complete list of metadata

Cited literature [40 references]  Display  Hide  Download
Contributor : Arnaud Eyraud Connect in order to contact the contributor
Submitted on : Friday, August 10, 2018 - 1:46:17 PM
Last modification on : Wednesday, April 27, 2022 - 4:36:40 AM
Long-term archiving on: : Sunday, November 11, 2018 - 1:03:05 PM


 Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed until : jamais

Please log in to resquest access to the document



Teuta Eljezi, Pierre Pinta, Damien Richard Richard, Jérémy Pinguet, Jean-Michel Chezal, et al.. In vitro cytotoxic effects of DEHP-alternative plasticizers and their primary metabolites on a L929 cell line. Chemosphere, Elsevier, 2017, 173, pp.452 - 459. ⟨10.1016/j.chemosphere.2017.01.026⟩. ⟨inserm-01856278⟩



Record views