Application of Millifluidics to Encapsulate and Support Viable Human Mesenchymal Stem Cells in a Polysaccharide Hydrogel

Abstract : Human adipose-derived stromal cells (hASCs) are widely known for their immunomodulatory and anti-inflammatory properties. This study proposes a method to protect cells during and after their injection by encapsulation in a hydrogel using a droplet millifluidics technique. a biocompatible, self-hardening biomaterial composed of silanized-hydroxypropylmethylcellulose (Si-HPMC) hydrogel was used and dispersed in an oil continuous phase. Spherical particles with a mean diameter of 200 µm could be obtained in a reproducible manner. The viability of the encapsulated hASCs in the Si-HPMC particles was 70% after 14 days in vitro, confirming that the Si-HPMC particles supported the diffusion of nutrients, vitamins, and glucose essential for survival of the encapsulated hASCs. The combination of droplet millifluidics and biomaterials is therefore a very promising method for the development of new cellular microenvironments, with the potential for applications in biomedical engineering.
Complete list of metadatas

Cited literature [63 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-01844534
Contributor : Valérie Pecqueret <>
Submitted on : Thursday, July 19, 2018 - 2:29:44 PM
Last modification on : Tuesday, July 23, 2019 - 5:14:03 PM
Long-term archiving on : Saturday, October 20, 2018 - 2:40:45 PM

File

ijms-19-01952-v2.pdf
Publisher files allowed on an open archive

Identifiers

Collections

Citation

Fabien Nativel, Denis Renard, Fahd Hached, Pierre-Gabriel Pinta, Cyril D’arros, et al.. Application of Millifluidics to Encapsulate and Support Viable Human Mesenchymal Stem Cells in a Polysaccharide Hydrogel. International Journal of Molecular Sciences, MDPI, 2018, 19 (7), pp.1952. ⟨10.3390/ijms19071952⟩. ⟨inserm-01844534⟩

Share

Metrics

Record views

175

Files downloads

146