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Journal articles
Comparative analysis of BRAF, NRAS and c-KIT mutation status between tumor tissues and autologous tumor cell-lines of stage III/IV melanoma
Abstract : In the last decade, advances in molecular biology have provided evidence of the genotypic heterogeneity of melanoma. We analysed BRAF, NRAS and c-KIT alterations in tissue samples from 63 stage III/IV melanoma patients and autologous cell-lines, using either allele-specific or quantitative PCR. The expression of BRAF V600E protein was also investigated using an anti-BRAF antibody in the same tissue samples. 81% of FFPE samples and tumor cell-lines harboured a genetic alteration in either BRAF (54%) or NRAS (27%) oncogenes. There was a strong concordance (100%) between tissue samples and tumor cell-lines. The BRAF V600E mutant-specific antibody showed high sensitivity (96%) and specificity (100%) for detecting the presence of a BRAF V600E mutation. The correlation was of 98% between PCR and immunohistochemistry results for BRAF mutation. These results suggest that BRAF and NRAS mutation status of tumor cells is not affected by culture conditions.
Cited literature [28 references]
https://www.hal.inserm.fr/inserm-01818897
Contributor : Elizabeth Bernardo <>
Submitted on : Tuesday, June 19, 2018 - 4:24:19 PM
Last modification on : Friday, June 19, 2020 - 3:08:53 AM
Long-term archiving on: : Tuesday, September 25, 2018 - 6:00:50 PM
Contributor : Elizabeth Bernardo <>
Submitted on : Tuesday, June 19, 2018 - 4:24:19 PM
Last modification on : Friday, June 19, 2020 - 3:08:53 AM
Long-term archiving on: : Tuesday, September 25, 2018 - 6:00:50 PM