Using Vitek MALDI-TOF mass spectrometry to identify species belonging to the Acinetobacter calcoaceticus–Acinetobacter baumannii complex: a relevant alternative to molecular biology?

Hélène Pailhories 1 Sophie Daure Matthieu Eveillard 1 Marie Joly-Guillou 1 Marie Kempf 1
1 CRCNA - Centre de Recherche en Cancérologie / Nantes - Angers
Abstract : Acinetobacter baumannii belongs to the Acinetobacter calcoaceticus-baumannii complex (Acb) containing 2 other pathogenic species: Acinetobacter pittii and Acinetobacter nosocomialis. Identification of these bacteria remains problematic despite the use of matrix-assisted laser ionization time-of-flight mass spectrometry (MALDI-TOF MS). Here, we enriched the SARAMIS™ database of the Vitek MS® plus mass spectrometer to improve the identification of species of the Acb complex. For each species, we incremented reference spectra. Then, a SuperSpectrum was created based on the selection of 40 specific masses. In a second step, we validated reference spectra and SuperSpectra with 100 isolates identified by rpoB gene sequencing. All the isolates were correctly identified by MALDI-TOF MS with the database we created as compared to the identifications obtained by rpoB sequencing. Our database enabled rapid and reliable identification of the pathogen species belonging to the Acb complex. Identification by MALDI-TOF MS with our database is a good alternative to molecular biology.
Keywords : Acinetobacter baumannii Acinetobacter pittii MALDI-TOF mass spectrometry SARAMIS™ SuperSpectrum Vitek MS® plus
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Journal articles
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https://www.hal.inserm.fr/inserm-01817635
Contributor : Elizabeth Bernardo <>
Submitted on : Monday, June 18, 2018 - 11:06:44 AM
Last modification on : Thursday, February 7, 2019 - 2:52:36 PM

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Hélène Pailhories, Sophie Daure, Matthieu Eveillard, Marie Joly-Guillou, Marie Kempf. Using Vitek MALDI-TOF mass spectrometry to identify species belonging to the Acinetobacter calcoaceticus–Acinetobacter baumannii complex: a relevant alternative to molecular biology?. Diagnostic Microbiology and Infectious Disease, Elsevier, 2015, 83 (2), pp.99 - 104. ⟨10.1016/j.diagmicrobio.2015.06.009⟩. ⟨inserm-01817635⟩

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