Single lipoaminoglycoside promotes efficient intracellular antibody delivery: A comprehensive insight into the mechanism of action - Archive ouverte HAL Access content directly
Journal Articles Nanomedicine: Nanotechnology, Biology and Medicine Year : 2018

Single lipoaminoglycoside promotes efficient intracellular antibody delivery: A comprehensive insight into the mechanism of action

(1, 2) , (2) , (2) , (2) , (2) , (2) , (3)
1
2
3

Abstract

Delivery of biologically active proteins into cells is emerging as important strategy for many applications. Previous experiments have shown that lipoaminoglycosides were capable of delivery of the anti-cytokeratin8 antibody (anti-K8) but only when formulated with lipid helpers potentially leading to toxicity from excess lipids. Here, we optimized anti-K8 delivery with various lipoaminoglycosides in the absence of a lipid helper. Results led to the identification of the aminoglycoside lipid dioleyl phosphoramido ribostamycin (DOPRI) as a potent intracellular delivery system for anti-K8. Electron microscopy revealed that delivered anti-K8 molecules were bound to intermediate filaments in cells. Anti-K8 was bound to the surface of DOPRI vesicles without perturbing lipid organization. Macropinocytosis and caveolin mediated endocytosis contributed to anti-K8 internalization and to filament labeling with a major contribution being made by the caveolin pathway. The results showed that the unique properties of DOPRI were sufficient for efficient intracellular protein delivery without requiring lipid helpers.
Embargoed file
Embargoed file
Ne sera jamais visible
Loading...

Dates and versions

inserm-01814772 , version 1 (13-06-2018)

Identifiers

Cite

Laurence Dallet, Marion Decossas, Jean-Christophe Taveau, Sophie Lecomte, Sylvie Poussard, et al.. Single lipoaminoglycoside promotes efficient intracellular antibody delivery: A comprehensive insight into the mechanism of action. Nanomedicine: Nanotechnology, Biology and Medicine, 2018, 14 (1), pp.141 - 151. ⟨10.1016/j.nano.2017.09.005⟩. ⟨inserm-01814772⟩
60 View
2 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More