Tropism and virulence of Cutibacterium (formerly Propionibacterium) acnes involved in implant-associated infection

Abstract : The recognition of the pathogenicity of Cutibacterium acnes in implant-associated infection is not always obvious. In this paper, we aimed to distinguish pathogenic and non-pathogenic C. acnes isolates. To reach this goal, we investigated the clonal complex (CC) of a large collection of C. acnes clinical isolates through Multi-Locus Sequence Typing (MLST), we established a Caenorhabditis elegans model to assess C. acnes virulence and we investigated the presence of virulence factors in our collection. Ours results showed that CC36 and CC53 C. acnes isolates were more frequently observed in prosthetic joint infections (PJI) than CC18 and CC28 C. acnes isolates (p = 0.021). The C. elegans model developed here showed two distinct virulence groups of C. acnes (p < 0.05). These groups were not correlated to CC or clinical origin. Whole genome sequencing allowed us to identify a putative gene linked to low virulent strains. In conclusion, MLST remains a good method to screen pathogenic C. acnes isolates according to their clinical context but mechanisms of C. acnes virulence need to be assess thought transcriptomic analysis to investigate regulatory process.
Document type :
Journal articles
Complete list of metadatas

Cited literature [34 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-01814725
Contributor : Elizabeth Bernardo <>
Submitted on : Wednesday, June 13, 2018 - 2:42:07 PM
Last modification on : Wednesday, May 29, 2019 - 1:56:01 PM

File

 Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed until : jamais

Please log in to resquest access to the document

Identifiers

Citation

Guillaume Aubin, Jean-Philippe Lavigne, Yohan Foucher, Sarah Dellière, Didier Lepelletier, et al.. Tropism and virulence of Cutibacterium (formerly Propionibacterium) acnes involved in implant-associated infection. Anaerobe, Elsevier Masson, 2017, 47, pp.73 - 78. ⟨10.1016/j.anaerobe.2017.04.009⟩. ⟨inserm-01814725⟩

Share

Metrics

Record views

172